Liver: cancer-metastasis-normal

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Category Human tissue array
   Cat. No. CSA
   Product description Liver: cancer-metastasis-normal
   Data sheet CSA2.pdf
CSA2.xls
   
  general_information
   No. of samples 59
   Text
(Old Cat. No: CS2) 
Current version: CSA2 
Primary cancer: 40 cores 
Metastatic cancer: 10 cores 
Non-neoplastic liver: 9 cores

Price : 5 slide for 600 $

CSA2  Human, liver cancer
               
  Age Sex Organ Diagnosis TNM Stage Months*
1 41 M liver HCC T3N0M0 ⅢA .
2 52 M liver HCC T3N0M0 ⅢA .
3 56 M liver HCC T3N0M0 ⅢA .
4 37 M liver HCC T4NxM1 .
5 32 M liver HCC T3N0M0 ⅢA .
6 57 M liver HCC T3N0M0 ⅢA .
7 65 M liver moderately differentiated HCC T2N0M0 .
8 72 M liver well & poorly differentiated HCC T2N0M0 .
9 58 F liver well differentiated HCC T2N0M0 .
10 52 M liver well differentiated HCC T2N0M0 .
11 40 M liver moderately differentiated HCC T2N0M0 .
12 67 M liver moderately differentiated HCC T2N0M0 .
13 20 M liver well differentiated HCC T4N0M0 ⅢB .
14 48 M liver moderately differentiated HCC T2N0M0 .
15 47 M liver HCC T1N0M0 .
16 69 F liver moderately differentiated HCC T2N0M0 .
17 63 F liver moderately differentiated HCC T2N0M0 .
18 60 F liver poorly differentiated HCC T2N0M0 .
19 66 F liver well differentiated HCC T2N0M0 .
20 64 M liver poorly differentiated HCC T3N0M0 ⅢA .
21 64 M liver moderately differentiated HCC T2N0M0 .
22 51 M liver moderately differentiated HCC T4N0M0 ⅢB .
23 65 F liver moderately differentiated HCC T2N0M0 .
24 41 M liver moderately differentiated HCC T2N0M0 .
25 57 F liver well differentiated HCC T2N0M0 .
26 57 M liver moderately differentiated HCC T3N0M0 ⅢA .
27 50 M liver poorly differentiated HCC T2N0M0 .
28 43 M liver moderately differentiated HCC T2N0M0 .
29 60 F liver poorly differentiated HCC T4N0M0 ⅢB .
30 64 M liver moderately differentiated HCC T3N1M0 ⅢC .
31 52 M liver moderately differentiated HCC T3N1M0 ⅢC .
32 54 M liver moderately differentiated HCC T4NxM1 .
33 56 M liver well differentiated HCC T3N0M0 ⅢA .
34 77 M liver moderately differentiated HCC T3N0M0 ⅢA .
35 38 M liver HCC T1NxM0 .
36 75 M liver moderately differentiated HCC T2N0M1 .
37 43 M liver moderately differentiated HCC T3N0M0 ⅢA .
38 72 M liver moderately differentiated HCC T4N1M0 ⅢC .
39 60 F liver cholangiocarcinoma T2N0M0 .
40 58 M liver cholangiocarcinoma T3N1M1 .
41 51 M colon metastatic HCC M1 .
42 62 M lung metastatic HCC M1 .
43 61 M lung metastatic HCC M1 .
44 60 M adrenal gland metastatic HCC M1 .
45 47 M lumbar vertebra metastatic HCC from #35 . . 22
46 75 M colon metastatic HCC from #36 . . 0
47 43 M soft tissue metastatic HCC from #37 . . 5
48 72 M lymph node metastatic HCC from #38 . . 0
49 60 F soft tissue metastatic cholangiocarcinoma from #39 . . 16
50 58 M lymph node metastatic cholangiocarcinoma from #40 . . 0
51 57 M liver normal liver (adjacent to cancer, match of #6) . . .
52 72 M liver normal liver (adjacent to cancer, match of #8) . . .
53 58 F liver normal liver (adjacent to cancer, match of #9) . . .
54 52 M liver normal liver (adjacent to cancer, match of #10) . . .
55 67 M liver normal liver (adjacent to cancer, match of #12) . . .
56 32 M liver normal liver (apart from cancer, match of #5) . . .
57 63 F liver normal liver (apart from cancer, match of #17) . . .
58 65 F liver normal liver (apart from cancer, match of #23) . . .
59 57 F liver normal liver (apart from cancer, match of #25) . . .
60 carbon            
               
HCC: hepatocellular carcinoma        
*: interval between primary and metastatic cancer      
TNM Classification : Liver carcinoma (AJCC 6th Edition)      

General Information of the Tissue Array Slide :

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area

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