Thyroid cancer

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Category Human tissue array
   Cat. No. CT
   Product description Thyroid cancer
   Data sheet CT1
CT1
   
  general_information
   No. of samples 59
   Text
Current version: CT1 
Thyroid cancer: 50 cores 
Normal thyroid: 9 cores

Price : 5 slide for 600 $

CT1  Human, thyroid cancer
             
  Age Sex Organ Diagnosis TNM Stage
1 38 F thyroid papillary carcinoma T4N1aM0
2 43 F thyroid papillary carcinoma T4N0M0
3 31 F thyroid papillary carcinoma T4N1aM0
4 35 F thyroid papillary carcinoma T2NxM0
5 41 F thyroid papillary carcinoma T4N0M0
6 57 F thyroid papillary carcinoma T4NxM0
7 52 F thyroid papillary carcinoma T2N0M0
8 45 M thyroid papillary carcinoma T4N0M0
9 40 F thyroid papillary carcinoma T4N0M0
10 60 F thyroid papillary carcinoma T4N1M0
11 48 F thyroid papillary carcinoma T4N0M0
12 29 F thyroid papillary carcinoma T2N1M0
13 72 M thyroid papillary carcinoma T4N1bM0
14 24 M thyroid papillary carcinoma T4N1M0
15 37 F thyroid follicular carcinoma T2N0M0
16 56 F thyroid papillary carcinoma T4N1bM0
17 35 M thyroid follicular carcinoma T3N0M0
18 55 F thyroid papillary carcinoma T4N0M0
19 35 F thyroid papillary carcinoma T4N0M0
20 54 M neck papillary carcinoma T4N1M0
21 28 F thyroid papillary carcinoma T4N1aM0
22 45 F thyroid papillary carcinoma T4N1M0
23 46 M thyroid papillary carcinoma T4N0M0
24 36 F thyroid papillary carcinoma T4N1M0
25 67 F thyroid papillary carcinoma T4N1M0
26 22 F thyroid follicular carcinoma T2N0M0
27 45 M thyroid papillary carcinoma T4N1M0
28 33 F thyroid follicular carcinoma T2N0M0
29 36 F thyroid papillary carcinoma T2NxM0
30 21 F thyroid papillary carcinoma T2N0M0
31 22 F thyroid papillary carcinoma T4N1aM0
32 18 F thyroid papillary carcinoma T4N1aM0
33 17 F thyroid papillary carcinoma T2N0M0
34 51 F thyroid papillary carcinoma T4N1M0
35 50 F neck papillary carcinoma    
36 57 F thyroid follicular carcinoma T2N0M0
37 38 F thyroid papillary carcinoma T4N1M0
38 20 F thyroid papillary carcinoma T4N1M0
39 27 F thyroid follicular carcinoma T2N0M0
40 65 M thyroid follicular carcinoma T3N0M0
41 40 F thyroid papillary carcinoma T4N0M0
42 69 F thyroid papillary carcinoma T4N1aM0
43 50 F thyroid papillary carcinoma T4N0M0
44 51 M thyroid follicular carcinoma T2N0M0
45 53 M thyroid poorly differentiated carcinoma T4N0M0
46 47 M thyroid papillary carcinoma T4N1M0
47 63 M thyroid papillary carcinoma T2N0M0
48 58 M thyroid papillary carcinoma T3N0M0
49 75 F thyroid papillary carcinoma T4N0M0
50 15 F thyroid papillary carcinoma T4N0M0
51 45 M thyroid benign thyroid tissue (same patient of #8) T4N0M0
52 31 F thyroid benign thyroid tissue T4N0M0
53 38 F thyroid benign thyroid tissue T4N0M0
54 50 F thyroid benign thyroid tissue (same patient of #43) T4N0M0
55 52 M thyroid benign thyroid tissue T2N1M0
56 78 F thyroid benign thyroid tissue T2NxM1
57 32 F thyroid benign thyroid tissue T4N1M0
58 15 F thyroid benign thyroid tissue (same patient of #50) T4N0M0
59 37 F thyroid benign thyroid tissue T2N0M0
60       carbon particle    
             
TNM and stage classification:thyroid carcinoma(AJCC 5th Edition)    

General Information of the Tissue Array Slide :

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area

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31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
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