Category Human tissue array
   Cat. No. BC
   Product description Normal organs and cancers
   Data sheet BC5
BC5
  general_information
   No. of samples 59
   Text
Current version : BC5 
No. of normal cores : 30 cores 
No. of cancer cores : 29 cores

Price : 5 slides for 600 $

General Information of the Tissue Array Slide

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis 

Storage and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.

Shaded area

1 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 19 20
21 22 23 24 25 26 27 28 29 30
31 32 33 34 35 36 37 38 39 40
41 42 43 44 45 46 47 48 49 50
51 52 53 54 55 56 57 58 59 60
BC5   Normal organs and cancers
         
  Age Sex organ Diagnosis
1 63 F skin normal
2 44 F breast normal
3 53 F spleen normal
4 55 M lymph node normal
5 57 M skeletal muscle normal
6 72 F lung normal
7 69 M salivary gland, sublingual normal
8 47 M liver normal
9 65 M gallbladder normal
10 73 F pancreas normal
11 24 M tonsil normal
12 56 M esophagus normal
13 31 M stomach, antrum normal
14 59 M stomach, fundus normal
15 52 F small bowel normal
16 60 M colon normal
17 54 M rectum normal
18 48 M kidney, cortex normal
19 48 M kidney, medulla normal
20 32 M bladder normal
21 32 M prostate normal
22 81 M testis normal
23 39 F uterine cervix normal
24 49 F endometrium normal, proliferative
25 42 F myometrium normal
26 35 F placenta normal
27 48 M adrenal normal
28 39 M thyroid normal
29 0 M brain normal
30 12 M cerebellum normal
31 57 M skin squamous cell carcinoma
32 64 F skin malignant melanoma
33 38 M subcutaneous tissue liposarcoma, myxoid
34 34 F breast ductal carcinoma in situ
35 43 F breast Infiltrating duct carcinoma
36 33 F lymph node Hodgkin's disease
37 53 F bone osteosarcoma
38 60 M lung adenocarcinoma
39 72 M lung squamous cell carcinoma
40 63 M liver cholangiocarcinoma
41 52 M liver hepatocellular carcinoma
42 35 F liver metastatic adenocarcinoma from rectum
43 56 M esophagus squamous cell carcinoma
44 68 F stomach adenocarcinoma
45 52 M stomach malignant lymphoma, B cell
46 51 F stomach signet ring cell carcinoma
47 61 M duodenum gastrointestinal stromal tumor, malignant
48 51 M sigmoid colon adenocarcinoma
49 73 M rectum adenocarcinoma
50 56 M kidney renal cell carcinoma
51 53 M bladder transitional cell carcinoma
52 70 M prostate adenocarcinoma
53 34 M testis seminoma
54 64 F uterine cervix squamous cell carcinoma
55 69 F endometrium endometrial carcinoma
56 42 F ovary metastatic adenocaricnoma from stomach
57 15 F ovary mucinous cystadenocarcinoma
58 47 F ovary papillary serous cystadenocarcinoma
59 69 F thyroid papillary carcinoma
60 carbon
 Category Human tissue array
   Cat. No. BB
   Product description Various cancers
   Data sheet BB4
BB4
  general_information
   No. of samples 59
   Text
Current version: BB4 
No. of cores: 59

Price : 5 slides for 600 $

BB4  Human various tumors
         
  Age Sex Organ Diagnosis
1 59 M skin malignant melanoma
2 25 F skin squamous cell carcinoma
3 50 F breast infiltrating duct carcinoma
4 57 F breast invasive papillary carcinoma
5 35 F breast infltrating lobular carcinoma
6 40 M lymph node malignant lymphoma, immunoblastic
7 58 M lymph node metastatic adenocarcinoma from stomach
8 53 F bone osteosarcoma
9 26 M bone giant cell tumor
10 40 M bone chondrosarcoma
11 51 F soft tissue liposarcoma
12 47 F soft tissue neurofibromatosis
13 74 M nasal cavity inverted papilloma
14 57 M larynx squamous cell carcinoma
15 60 M lung adenocarcinoma
16 51 F lung squamous cell carcinoma
17 68 F lung adenocarcinoma
18 60 M lung small cell carcinoma
19 88 F tongue squamous cell carcinoma
20 34 F parotid gland pleomorphic adenoma
21 50 F parotid gland Warthin tumor
22 40 F parotid gland pleomorphic adenoma
23 56 M submandibular gland salivary duct carcinoma
24 69 F liver cholangiocarcinoma
25 51 M liver metastatic gastric cancer
26 64 M liver hepatocellular carcinoma
27 62 F gall bladder adenocarcinoma
28 64 F pancreas adenocarcinoma
29 68 M esophagus squamous cell carcinoma
30 73 M stomach adenocarcinoma, poorly differentiated
31 63 M stomach adenocarcinoma, moderately differentiated
32 59 F stomach signet ring cell carcinoma
33 62 M stomach malignant lymphoma
34 51 M stomach borderline stromal tumor
35 42 M small intestine malignant stromal tumor
36 52 F appendix pseudomyxoma peritonia
37 53 M colon adenocarcinoma
38 67 M rectum adenocarcinoma
39 75 F kidney transitional cell carcinoma
40 54 F kidney renal cell carcinoma
41 75 F kidney renal cell carcinoma
42 65 M urinary bladder poorly differentiated carcinoma
43 67 M urinary bladder transitional cell carcinoma, high grade
44 62 M prostate adenocarcinoma
45 30 M testis seminoma
46 68 F uterus endometrial adenocarcinoma
47 57 F uterus leiomyosarcoma
48 45 F uterus leiomyoma
49 63 F uterine cervix squamous cell carcinoma
50 12 F ovary endodermal sinus tumor
51 33 F ovary mucinous adenocarcinoma
52 70 F ovary fibrothecoma
53 67 F adrenal gland cortical carcinoma
54 61 F adrenal gland pheochromocytoma
55 54 M thyroid papillary carcinoma
56 58 F thyroid minimaly invasive follicular carcinoma
57 74 M thymus thymoma
58 66 F brain meningioma
59 62 M brain glioblastoma multiforme
60 64 F lymph node malignant melanoma

General Information of the Tissue Array Slide

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol)
  • Storage and stability
  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times - Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • Refer to data sheet for slide orientation.
Category Human tissue array
   Cat. No. BA
   Product description Evaluation slide (TEST SLIDE)
   Data sheet BA3
BA3
  general_information
   No. of samples 24
   Text
Current version: BA3 
No. of normal cores: 12 cores 
No. or cancer cores: 12 cores 
The price of the TEST SLIDE is one-fourth of regular array slide. The quality of this test slide is same as the other array slides. This slide can be used for adjustment of experimental condition. The TEST SLIDE is also useful for most researchers who consider the experiment using tissue array slides.

Price : 5 slides for 600 $

BA3,  Test slide (normal organs and tumors)
         
No. Age Sex Organ Diagnosis
1 36 F skin normal
2 37 F breast normal
3 63 M spleen normal
4 67 M skeletal muscle normal
5 60 M lung normal
6 42 F liver normal
 
7 41 M stomach , body normal
8 53 M colon normal
9 35 M kidney normal
10 70 M prostate normal
11 30 F placenta normal
12 0 F brain normal
 
13 26 F breast infiltrating duct carcinoma
14 59 M lung squamous cell carcinoma
15 54 M liver hepatocellular carcinoma
16 52 M esophagus squamous cell carcinoma
17 49 M stomach adenocarcinoma
18 39 M small bowel malignant stromal tumor
 
19 57 F rectum adenocarcinoma
20 44 F kidney renal cell carcinoma
21 56 F urinary bladder transitiomal cell carcinoma
22 67 F uterus endometrial carcinoma
23 34 F ovary mucinous cystadenocarcinoma
24 64 F lymph node metastatic malignant melanoma

General Information of the Tissue Array Slide

 

Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis (Recommended procedures in www.tissue-array.com -> protocol) Storage

and stability

  • Individual slide is paraffin-coated and put in an air-tight pack with inert gas.
  • Tissue array slides are shipped in room temperature.
  • If the slides are stored in 4 degree, they are good up to one year.

How processed

  • Tissues were initially fixed with formalin except for some of the animal tissues.
  • Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
  • Cleared by xylene, three changes for 1 hour each.
  • Infiltrated with 60°C paraffin, three changes for 1 hour each
  • Microtoming in 4 µm thickness and put on silane coating slide from Muto Pure Chemicals (Japan)

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes

Slide orientation

  • Refer to data sheet for slide orientation.
Category Human tissue array
   Cat. No. AA
   Product description Normal organs
   Data sheet AA9
AA9
  general_information
   No. of samples 59
   Text
Current version: AA9 
Various normal organs: 59 cores

Price :  5 slides for 600 $

General Information of the Tissue Array Slide Applications

  • Immunohistochemistry
  • In situ hybridization, RNA or DNA
  • Fluorescent in situ hybridization (FISH)
  • In situ PCR, RNA or DNA
  • TUNEL for apoptosis

Storage and stability

Cat. No. Product description No. of samples
Human tissue array
AA
Normal organs  
59
BA
Evaluation slide (TEST SLIDE)  
24
BB
Various cancers  
59
BC
Normal organs and cancers  
59
CA
prostate cancer  
49
CBA
Breast: cancer-metastasis-normal  
60
CC
lung cancer  
59
CCA
Lung: cancer-metastasis-normal  
59
CCN
Normal lung tissue  
59
CD
colon and rectum cancer  
59
CDA
colorectal: cancer-metastasis-normal  
59
CDN
Normal colon and rectum tissue  
59
CH
larynx and pharynx cancer  
60
CJ
Ovary cancer  
59
CL
Kidney cancer  
59
CQ
Stomach cancer  
59
CQN
Normal stomach tissue  
59
CR
Esophagus cancer  
60
CS
Liver cancer  
59
CSA
Liver: cancer-metastasis-normal  
59
CSN
Non-neoplastic liver tissue  
59
CT
Thyroid cancer  
59
CW
Lipomatous tumors  
59
CX
Skin cancer  
59
CYN
Normal endometrium  
59
CZA
Uterine cervix: cancer-metastasis-normal  
59
DAA
Gastrointestinal stromal tumor: primary-metastasis-normal  
59
FA
Metastatic cancer to ovary  
59
FB
Primary cancer of FA  
30
MA
Common cancer (1)  
59
MAN
Normal matching tissue of MA  
59
MB
Common cancer (2)  
59
MBN
Normal matching tissue of MB  
59
MC
Common cancer (3)  
59
MCN
Normal matching tissue of MC  
59
VA
Varous cancers-high density(1)  
146
VB
Various cancers-high density(2)  
146
VC
Various cancers-high density(3)  
146
Fetal tissue array
HA
14-35 weeks (1): skin, spleen, muscle, lung, heart, tongue  
60
HB
14-35 weeks (2): liver, pancreas, stomach, intestines, kidney  
60
HC
14-35 weeks (3): placenta, adrenal, thymus, brain  
60
HD
14-18 weeks  
60
HE
19-22 weeks  
60
HF
24-26 weeks  
60
HG
28-31 weeks  
60
HH
33-35 weeks  
60
Cell line array
XA
human gastric cancer cell lines  
32
XB
Human colorectal cancer cell lines  
58
XC
Human liver cancer cell lines  
20
XD
Human gastric cancer cell lines grown in nude mice  
34
XF
Human liver cancer cell lines grown in nude mice  
28
Animal tissue array
ZC
Normal organs of adult rats, formalin fixed  
23
ZD
Normal organs of adult rats, formalin fixed, 4.0mm  
23
ZE
Normal organs of adult mice, formalin fixed  
24
ZF
Normal organs of adult rats, perfusion fixation  
59
ZG
Normal organs of adult mice, perfusion fixation  
59
  • Classical

DMEM LOW GLUCOSE (1g/l)                                        MEDIUM 199 WITH HANKS’ SALTS

DMEM HIGH GLUCOSE (4,5 g/l)                                    MEM ALPHA MODIFICATION

DMEM / HAM’S F-12 MIX (1:1)                                      GLASGOW´S MEM

HAM’S F-10 AND HAM’S F-12                                        MINIMAL ESSENTIAL MEDIUM EAGLE (MEM)

ISCOVE’S MODIFIED DMEM (IMDM)                                RPMI 1640

LEIBOVITZ’S L-15                                                       MEDIUM 199 WITH EARLE’S SALTS

 

DMEM LOW GLUCOSE (1g/l)    
       
  SIZE CATALOGUE
NUMBER
USD
DMEM (1g/l) liquid
without L-Glutamine
10X500 ml E15-005 85$
DMEM (1g/l) liquid
with L-Glutamine
10X500 ml E15-806 85$
DMEM (1g/l) liquid
with 25mM HEPES
without L-Glutamine
10X500 ml E15-007 90$
DMEM (1g/l) liquid
with HEPES
with L-Glutamine
10X500 ml E15-808 90$
       
       
DMEM HIGH GLUCOSE(4,5 g/l)  
       
  SIZE CATALOGUE
NUMBER
USD
DMEM (4,5g/l) liquid
without L-Glutamine
10X500 ml E15-009 85$
DMEM (4,5g/l) liquid
with L-Glutamine
10X500 ml E15-810 85$
DMEM (4,5g/l) liquid
with Sodium Pyruvate
without L-Glutamine
10X500 ml E15-011 90$
DMEM (4,5g/l) liquid
with Sodium Pyruvate
with L-Glutamine
10X500 ml E15-843 90$
       
       
DMEM / HAM’S F-12 MIX (1:1)  
       
  SIZE CATALOGUE
NUMBER
USD
DMEM/Ham’s F-12 liquid
without L-Glutamine
10x500 ml E15-012 85$
DMEM/Ham’s F-12 liquid
with L-Glutamine
10x500 ml E15-813 85$
       
       
HAM’S F-10 AND HAM’S F-12    
       
  SIZE CATALOGUE
NUMBER
USD
Ham’s F-10 liquid
without L-Glutamine
10x500 ml E15-014 85$
Ham’s F-10 liquid
with L-Glutamine
10x500 ml E15-815 85$
Ham’s F-12 liquid
without L-Glutamine
10x500 ml E15-016 85$
Ham’s F-12 liquid
with L-Glutamine
10x500 ml E15-817 85$
       
       
ISCOVE’S MODIFIED DMEM (IMDM)  
       
  SIZE CATALOGUE
NUMBER
USD
IMDM liquid
without L-Glutamine
10x500 ml E15-018 95$
IMDM liquid
with L-Glutamine
10x500 ml E15-819 95$
       
       
LEIBOVITZ’S L-15      
       
  SIZE CATALOGUE
NUMBER
USD
Leibovitz’s L-15 liquid
without L-Glutamine
10x500 ml E15-020 95$
Leibovitz’s L-15 liquid
with L-Glutamine
10x500 ml E15-821 95$
       
       
MEDIUM 199 WITH EARLE’S SALTS  
       
  SIZE CATALOGUE
NUMBER
USD
Medium 199 liquid
with Earle's Salts
without L-Glutamine
10x500 ml E15-033 95$
Medium 199 liquid
with Earle's Salts
with L-Glutamine
10x500 ml E15-834 95$
       
       
MEDIUM 199 WITH HANKS’ SALTS  
       
  SIZE CATALOGUE
NUMBER
USD
Medium 199 liquid
with Hank's Salts
with HEPES
without L-Glutamine
10x500 ml E15-037 95$
Medium 199 liquid
with Hank's Salts
with HEPES
with L-Glutamine
10x500 ml E15-838 95$
       
       
MEM ALPHA MODIFICATION    
       
  SIZE CATALOGUE
NUMBER
USD
MEM Alpha Mod. liquid
without Nucleosides
with L-Glutamine
10x500 ml E15-832 195$
MEM Alpha Mod. liquid
with Nucleosides
with L-Glutamine
10x500 ml E15-862 265$
       
       
GLASGOW´S MEM      
       
  SIZE CATALOGUE
NUMBER
USD
Glasgow's MEM
with L-Glutamine
10x500 ml E15-829 172.5$
       
       
MINIMAL ESSENTIAL MEDIUM EAGLE (MEM)  
       
  SIZE CATALOGUE
NUMBER
USD
MEM liquid
with Earle's Salts
without L-Glutamine
10x500 ml E15-024 85$
MEM liquid
with Earle's Salts
with L-Glutamine
10x500 ml E15-825 85$
       
       
RPMI 1640      
       
  SIZE CATALOGUE
NUMBER
USD
RPMI 1640 liquid
without L-Glutamine
10x500 ml E15-039 85$
RPMI 1640 liquid
with L-Glutamine
10x500 ml E15-840 85$
RPMI 1640 liquid
with HEPES
without L-Glutamine
10x500 ml E15-041 95$
RPMI 1640 liquid
with HEPES
with L-Glutamine
10x500 ml E15-842 95$
RPMI 1640 liquid
without L-Glutamine
without Phenol Red
10x500 ml E15-048 95$
RPMI 1640 liquid
with L-Glutamine
without Phenol Red
10x500 ml E15-848 95$
  • Special

Cell Type Specific                                                    READY MIX

Animal-derived component free                                THERMOSTABLE MEDIA

 

Cell Type Specific      
       
  SIZE CATALOGUE
NUMBER
USD
Quantum 333
Complete Fibroblast Medium,
with L-Glutamine
10x500 ml U15-813 175$
Quantum 101
Complete HeLa Medium,
with L-Glutamine
10x500 ml U15-814 175$
Quantum 263
Complete Tumor Cell Medium,
with L-Glutamine
10x500 ml U15-815 175$
Quantum 286
Complete Epithelial Medium,
with L-Glutamine
10x500 ml U15-818 175$
Quantum 3-21
Complete Amnion Medium,
with L-Glutamine
10x100 ml U11-020 395$
Quantum PBL
Complete Lymphocyte Medium,
with L-Glutamine
10x100 ml U11-022 265$
       
       
  SIZE CATALOGUE
NUMBER
USD
Endothelial Medium,
without L-Glutamine
10x500 ml U15-002 205$
Endothel Base Medium 
MCDB 131
without L-Glutamine
10x500 ml U15-011 155$
Endothel Supplement, frozen
with Growth Factors & Heparin, 
without Antibiotics
10x50 ml U05-012 145$
Macrophage Medium Mouse,
without L-Glutamine
10x500 ml U15-005 295$
       
       
Animal-derived component free  
       
  SIZE CATALOGUE
NUMBER
USD
Hybridoma Express
without L-Glutamine
10x500 ml U15-001 295$
VERO Express
with L-Glutamine
10x500 ml D15-807 235$
CHO Express
with L-Glutamine
10x500 ml D15-808 245$
MDCK Express
with L-Glutamine
10x500 ml D15-809 235$
HEK 293 Express
with L-Glutamine
10x500 ml D15-810 245$
InsectExpress Sf9-S2,
with L-Glutamine
10x500 ml E15-875 295$
TC 100 Insect Media
with L-Glutamine
10x500 ml E15-856 205$
       
       
READY MIX      
       
  SIZE CATALOGUE
NUMBER
USD
RPMI 1640 READY MIX 10x500 ml R15-802 175$
DMEM READY MIX  10x500 ml R15-801 175$
DMEM READY MIX
with Sodium Pyruvate
10x500 ml R15-803 185$
Ham´s F10 READY MIX
with 20% FBS
with Gentamicin
10x100 ml R11-821 205$
       
       
THERMOSTABLE MEDIA    
       
  SIZE CATALOGUE
NUMBER
USD
DMEM (TS) (1g/l) liquid
Thermostable Medium
without L-Glutamine
10x500 ml E15-080 120$
DMEM (TS) (4,5g/l) liquid
Thermostable Medium
without L-Glutamine
10x500 ml E15-082 120$
RPMI 1640 (TS) liquid
Thermostable Medium
without L-Glutamine
10x500 ml E15-084 120$

For USA call : 888-892-8408

Cell Culture Products
  SFM Screening Kit
  The SFM Screening Kit is intended for researchers seeking to identify the most appropriate SFM formulation for a specific cell type.  The kit contains a 100 ml sample of each of five serum-free media including: BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™, DMEM/F12, and IMDM along with 25 ml of FNC COATING MIXâ.  Each of the media are complete, ready-to-use.
   
  Storage Store at 4° C  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
415 SFM Screening Kit Liquid, Ready to Use 1 kit $175.00  
   
  BRFF-BMZERO™ is a complete serum-free medium designed to grow human breast cells.3  This SFM was originally used for establishing new epithelial cell lines from explants of human breast tissue and to grow immortalized normal breast cell lines.  For optimum cell attachment, use FNC COATING MIX®.  
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
           
401 BRFF-BMZERO™ Liquid, Ready to Use 500 mL $60.00  
   
  BRFF-EPM2™ is a complete serum-free medium designed to grow human epidermal-like cells.4  This SFM formulation was originally optimized for human esophageal epithelial cells.  Explant cultures of human skin in BRFF-EPM2 yields outgrowths of epithelial cells.  This medium is also useful for culturing a number of human cancer cell lines.  For optimum cell attachment, use FNC COATING MIX®.  
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
402 BRFF-EPM2™ Liquid, Ready to Use 500 mL $60.00  
   
  BRFF-HPC1™ is a complete serum-free medium designed for establishing new cell lines from human prostate tissue.5  Epithelial cell lines, from both benign prostatic hyperplasia and prostatic carcinoma, have been established and maintained in this medium.  This medium contains dihydrotestosterone.  For optimum cell attachment, use FNC COATING MIX®
  Storage Store at -70° C, supplied frozen   
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
403 BRFF-HPC1™ Liquid, Ready to Use 500 mL $60.00  
  BRFF-P4-8F™
  BRFF-P4-8F™ was designed to grow the immortalized normal prostatic cell line 267-B1.6  It also supports the growth of certain established human prostatic cancer cell lines such as PC-3.  For optimum cell attachment, use FNC COATING MIX®.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
404 BRFF-P4-8F™ Liquid, Ready to Use 500 mL $60.00  
  DMEM/F12 SFM
  DMEM/F12 is a serum-free medium formulation for general use.  This formulation is a 1:1 blend of DMEM and Ham’s F12 media supplied complete, ready-to-use with L-glutamine, Hepes, BPE and EGF for culturing a wide range of cell types.  Contains no phenol red or antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
410 DMEM/F12 Liquid, Ready to Use 500 mL $30.00  
   
  Iscove’s Modified Dulbecco’s Medium (IMDM) is a standard serum-free medium.  It is supplemented with BPE and EGF and contains L-glutamine and Hepes but no phenol red or antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.2-7.5 (5% CO2 atmosphere is recommended for culture)  
Osmolality 270 -300 mOsM  
     
411 IMDM Liquid, Ready to Use 500 mL $30.00  
   
  PET is a cell dissociation mix specially formulated for use with the "Cold Trypsin" method.  When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent will yield more viable cells from monoloyers grown in serum-free medium.   The solution contains polyvinylpyrrolidone, EGTA and trypsin in a HBS base.
 
Suggested Use:  After rinsing the cell monolayers using HBS, add 3 ml of ice cold PET dissociation reagent.  Do not keep the flask in the incubator.  Incubate at room temperature and monitor the monolayer by microscopy (preferably phase contrast).  When the cells begin to separate, gently remove most of the PET (without removing the cells) and allow the flask to remain at room temperature.  To determine whether the cells are ready to harvest, tap the flask (held in an upright position) firmly by hand.  When the cells float free, re-suspend them in cold HBS supplemented with 5% Fetal Bovine Serum to stop the tryptic action.  Mix well by pipetting the cell suspension, centrifuge and re-suspend the cell pellet in the appropriate complete serum-free medium.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 4 - 6 weeks  
pH 7.4-7.6  
Osmolality 270 -300 mOsM  
     
405 PET  Liquid, Ready to Use 100 mL $45.00  
   
  Freezing Media Pair are used to cryopreserve suspensions of viable cells detached from monolayer cultures using the "cold trypsin" method with PET.  Cells which have been cryopreserved in this medium have been shown to have superior viability upon reconstitution.  The Freezing Media Pair has been used extensively to cryopreserve human cells grown in serum-free media.
 
Suggested Use:  To cryopreserve cells, centrifuge the cell suspension.  Re-suspend the cells at 4 x 106 cells/ml in ice-cold Freezing Medium A.  Slowly add an equal volume of ice-cold Freezing Medium B to the cell suspension.  Mix well.  Transfer 1 ml aliquots of the cell suspension to  pre-cooled cryovials.  Freeze slowly using an automatic controlled rate (1°C per minute) freezer to -90°C.  Alternatively, place the cryovials inside a Styrofoam rack and place the rack in a -80°C mechanical freezer for 24 hours.  Transfer the frozen vials to a liquid nitrogen freezer (vapor phase) for permanent storage.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 3 months  
pH 7.7-7.9 for a 1:1 mixture of Media A and Media B  
     
406 Freezing Media Pair Liquid, Ready to Use 50 mL $30.00  
   
  FNC COATING MIX® is formulated to dramatically increase the attachment of cells to the plastic growth substratum used for culturing mammalian cell lines.  Many fastidious cell types such as human prostate and breast epithelial cells plated in the appropriate serum-free medium will attach and grow more effectively on FNC-coated plastic surfaces.
 
Suggested Use:  To coat the growth surface, add 0.2 ml FNC COATING MIX® per square centimeter of surface area of the culture vessel (for example use 5 ml for a T-25 flask).  The surface should be completely covered with a layer of liquid.  Incubate for 30 seconds and remove the coating mix with a pipet.  Residual FNC COATING MIX® will not adversely affect the viability of the cells to be plated.  Do not return any “used” coating mix to the original container of FNC.  This will compromise the performance of the product.  The coating can be done immediately before seeding the cells or up to one hour beforehand.
  Storage Store at 4° C, FNC COATING MIX® should not be frozen  
Stability at 4° C 2 years  
pH 7.1-7.4  
Osmolality 280 -300 mOsM  
     
407 FNC COATING MIX® Liquid, Ready to Use 50 mL $70.00  
   
  Hepes Buffered Saline with or without Phenol Red is an isotonic saline buffered with Hepes.  It is used for rinsing cell monolayers prior to cell dissociation using PET or for washing harvested cells.
  Storage Store at 4° C, shipped at room temperature  
Stability at 4° C 2 years  
pH 7.2-7.5  
Osmolality 270 -300 mOsM  
     
408 HBS w/ Phenol Red Liquid, Ready to Use 500 mL $16.00  
409 HBS w/out Phenol Red Liquid, Ready to Use 500 mL $16.00  
   
  Bovine Pituitary Extract prepared from TSE-free animals.  The extracts were prepared from pituitaries harvested from animals in the United States.  The pituitaries were obtained only from USDA inspected facilities where the animals receive pre- and post-mortem inspection and were found free of contagious diseases.  Foot-and-mouth disease, rinderpest, and contagious bovine plUSDpneumonia are not present in U.S. herds.  Further, there are no known cases of bovine spongiform encephalopathy (BSE) in the herds from which the pituitaries were obtained.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C not recommended  
Protein Concentration: 9-12 mg/ml by Bradford Assay  
     
413 Bovine Pituitary Extract Liquid, Ready to Use 100 mg $75.00  
414 Bovine Pituitary Extract Liquid, Ready to Use 250 mg $169.00  
  Bovine Pituitary Extract Liquid, Ready to Use bulk inquire  
  CeHR Medium
  CeHR Medium is a defined medium for the cultivation of the nematode Caenorhabditis elegans.  The medium is supplied ready-to-use containing the requisite salts, minerals, vitamins, growth factors, nucleic acids, and amino acids.  Non-fat milk is added and is without antibiotics.
  Storage Store at -70° C, supplied frozen  
Stability at 4° C 1-2 weeks  
     
412 CeHR Medium Liquid, Ready to Use 500 mL $120.00  

For USA call : 888-892-8408

Product Code Product Name Price
                        Cell Culture Products 1 - 9 Units
401 BRFF-BMZERO™ $60.00
402 BRFF-EPM2™ $60.00
403 BRFF-HPC1™ $60.00
404 BRFF-P4-8F™ $60.00
405 PET™ $45.00
406 Freezing Media Pair $30.00
407

FNC Coating Mix

$70.00
408 HBS w/ Phenol Red $16.00
409 HBS w/out Phenol Red $16.00
410 DMEM/F12-1:1 $30.00
411 IMDM $30.00
412 CeHR $120.00
413 Bovine Pituitary Extract, 100mg $75.00
414 Bovine Pituitary Extract, 250 mg $169.00
415 SFM Screening Kit $175.00
Human Epidermal Melanocytes, (HEM)

Cryopreserved Cells

 

Proliferating Cultures

Catalog Number: amp-106-5C

 

 Catalog Number: amp-106-25P

 > 550,000 viable cells/vial

 

Tertiary cultures established from cryopreserved cells, grown to approximately 55% confluence in 3 x T25 flasks

  *NEW:

Instructions for storage, initiation of cultures from cryopreserved cells, and subculture

 

Product Description
HeM are human melanocytes isolated from foreskin of healthy children and provided as such. Each vial contains >550,000 viable cells that have been cryopreserved between the end of the secondary and terrtiarly culture stage in a medium containing 11% dimethylsulfoxide (DMSO) and 51% fetal bovine serum. I

Each lot of cells is performance tested by culturing the cells through multiple passages HAM-F10 nutrient mixture supplemented with HM-GSM in the absence of antibiotics and antimycotics. During this culture period, no contamination by bacteria, yeast, or fungi was detected.

Upon thawing, the cells are guaranteed to be >75% viable and to have a potential of >17 population doublings when handled according to the directions provided in this document.

HEM Human Melanocytes

   

Intended Use
Cryopreserved HeM are intended for research use only. Not for use in, humans, or diagnostic procedures.

Storage and Stability
We shhip the cryopreserved HeM  frozen on dry ice. The cells should be stored in the vapor phase of a liquid N2 freezer. If liquid N2 enters the vial, it may explode at roomtemperature.

The viability of cryopreserved cells will decreases after 2 years in storage in N2 or 2 months at -85°

Initiating cultures from cryopreserved cells


Caution: Although cryopreserved cells or donors have been tested, they are potentially pathogenic or biohazardous materials.

We recommend seeding cells recovered from N2 at a density of 4,500 viable cells/cm2. For example, four 25 cm2 tissue culture flasks can usually be established from one vial containing >550,000 HeM.

Procedure

1. Prepare a beaker of water at 37° C. Place the MelanoMax TM supplemented culture medium in a 37° C bath.

2. Remove a vial of HeM from N2 storage, taking care to protect hands.

3. Take out the vial from N2 and visually verify the absence of N2 inside the vial and open the vial leaving the cap on the vial, dip the vial up to 1/4 of its height in a liquid nitrogen bath and wait until the evaportion of the liquid (about 15 min), then tighten the cap.

4. Dip the 1/2 of the vial into a 37° C water bath to thaw. You can  open the vial under a laminar flow and thaw the cells by adding an amount of MelanoMax TM supplemented culture medium already brought to 37°C.

5. Open the vial and use a 1 ml pipette to disperse the cells.

6. Remove 20 µl from the vial and dilute the cell suspension in 20 µl of trypan blue solution.

7. Using a hemacytometer, determine the number of viable cells per ml.

8. Use HAM F10 supplemented with HM-GMS (cat. # GENT-1615-15) or MelanoMax TMsupplement , Antibiotics, HEPES 6mM, Foetal Bovine Serum 5-10%  to dilute the contents of the vial (1 ml) to a concentration of 26,000 viable cells/ml.

9. Add 5 ml of cell suspension to each 25 cm2 culture flask or put the entire vial contant in 50 ml adequate MelanoMax TM supplemented culture medium and transfer to a 175 cm2 culture flask.

10. Following inoculation, swirl the MelanoMax TM supplemented medium in the flasks to distribute the cells.

11. Incubate the cultures in a 37° C, 5% CO2/95% air, 100% humidified cell culture incubator. Do not disturb the culture for at least 25 hours after the culture has been initiated.

Maintenance of stock cultures

1. Change the culture medium to fresh MelanoMax TM supplemented supplemented HAM F10, 24 to 36 hours after establishing a culture from cryopreserved cells. For subsequent subcultures change the medium 49 hours after establishing the subculture. For best results and highest growth rates, warm the medium to 37° C before use.

2. Change the MelanoMax TM supplemented medium every other day thereafter, until the culture is approximately 88% confluent.

3. Once the culture reaches 88% confluence, change the MelanoMax TM supplemented medium every day.

4. It is possible to add 30 nM TPA (phorbol 12-myristate 13-acetate) and 2nM cholera toxin to the culture medium if you are not using MelanoMax TM supplement.

5. To further accelerate cell growth, one may add to the same culture medium 45µg/ml bovine pituitary extract and 0.6-6ng/ml EGF.  Pituary extract lyopilised for melnocyte culture: 35 Euro /1 mg

Notes: To achieve the highest cell densities, the culture medium should be changed every day as the cultures approach confluence. The number of passages that can be achieved will vary with the starting cell density and the methods employed.

HeM cultures seeded at 5,500 cells/cm2 from N2 preserved cells should reach 88% confluence in 8 days. In this culture, most of the cells should have a healty morphology when the cultures are sparse. As the cultures become dense, the cells may begin to become more bipolar and aligned in patterns. Some irregularly sized and shaped cells may be observed. Occasionally, small numbers of keratinocytes persist in the tertiary culture. Keratinocytes do not readily proliferate in medium supplemented withMelanoMax TM supplemented or HM-GMS and should be virtually absent in subsequent cultures.

Subculture of HeM
View the culture under a microscope to ascertain the condition of the culture (ie., confluence, mitotic activity). This protocol is designed for the subculture of one 25 cm2 culture flask. If different sized culture vessels are to be used, reagent volumes should be adjusted accordingly.

1. Assemble subculture reagents and materials:

HAM- F10  (4 Euro/ 500ml) supplemented with HM-GMS or MelanoMax TM supplemented 
Trypsin/EDTA solution
Trypsin Neutralizer solution
Culture vessels 
Sterile pipettes 
Sterile 15 ml conical tubes


Tip:  1.The MelanoMax TM supplemented medium should be warmed to 37° C prior to use. We do NOT recommend warming the other reagents prior to use.
2. Remove all of the culture medium from the flask and 4 ml of Trypsin/EDTA solution to the flask. Rock the flask to ensure that the entire surface is covered.
3. Immediately remove the Trypsin/EDTA solution.
4. Incubate the flask at room temperature for approximately 2 minutes.
5. View the culture under a microscope. Continue the incubation until the cells have become nearly completely round with a few small processes remaining.
6. Rap the flask gently to dislodge cells from the surface.
7. Add 3 ml of Trypsin Neutralizer solution to the flask and transfer the detached cells to a sterile 15 ml conical tube.
8. Add 3 ml additional Trypsin Neutralizer solution to the flask and pipette the solution over the flask surface several times to remove any remaining cells. Add this solution to the 15 ml conical tube.
9. Centrifuge the cells at 170 x g for 8 minutes. Observe the cell pellet.
10. Remove the supernatant from the tube slill containing cell pellet.
11. Resuspend the cell pellet in 4 ml MelanoMax TM supplemented Medium HAM-F10. Pipette the cells up and down with a 10 ml pipette to ensure a homogeneous cell suspension.
12. Determine the concentration of cells.
13. Dilute the cells in MelanoMax TM supplemented HAM F10 and seed new culture vessels with 5,500 cells/cm2.
14. Incubate the cultures in a 37° C, 5% CO2/95% air, humidified cell culture incubator.

Tip: Do not dammage your HeM during trypsinization by exposure to the Trypsin/EDTA solution for ceveral hours, trypsinization at temperatures higher than 22° C. Check to make sure that the temperature of trypsinization is appropriate and, if necessary, alter the incubation time. Cellular damage by centrifugation can be debris of cells  that do not pellet in the bottom of the tube because of the presence of free DNA and the cells will be lost upon pipetting the supernatant. In that case viable cells have to be rescued by pipetting the DNA cell suspention up and down in a 10 ml pipette to shear the DNA, and centrifuging the suspension again to recover the pellet.

Cat. No. Product description No. of samples
Human tissue array
AA
Normal organs  
59
BA
Evaluation slide (TEST SLIDE)  
24
BB
Various cancers  
59
BC
Normal organs and cancers  
59
CA
prostate cancer  
49
CBA
Breast: cancer-metastasis-normal  
60
CC
lung cancer  
59
CCA
Lung: cancer-metastasis-normal  
59
CCN
Normal lung tissue  
59
CD
colon and rectum cancer  
59
CDA
colorectal: cancer-metastasis-normal  
59
CDN
Normal colon and rectum tissue  
59
CH
larynx and pharynx cancer  
60
CJ
Ovary cancer  
59
CL
Kidney cancer  
59
CQ
Stomach cancer  
59
CQN
Normal stomach tissue  
59
CR
Esophagus cancer  
60
CS
Liver cancer  
59
CSA
Liver: cancer-metastasis-normal  
59
CSN
Non-neoplastic liver tissue  
59
CT
Thyroid cancer  
59
CW
Lipomatous tumors  
59
CX
Skin cancer  
59
CYN
Normal endometrium  
59
CZA
Uterine cervix: cancer-metastasis-normal  
59
DAA
Gastrointestinal stromal tumor: primary-metastasis-normal  
59
FA
Metastatic cancer to ovary  
59
FB
Primary cancer of FA  
30
MA
Common cancer (1)  
59
MAN
Normal matching tissue of MA  
59
MB
Common cancer (2)  
59
MBN
Normal matching tissue of MB  
59
MC
Common cancer (3)  
59
MCN
Normal matching tissue of MC  
59
VA
Varous cancers-high density(1)  
146
VB
Various cancers-high density(2)  
146
VC
Various cancers-high density(3)  
146
Fetal tissue array
HA
14-35 weeks (1): skin, spleen, muscle, lung, heart, tongue  
60
HB
14-35 weeks (2): liver, pancreas, stomach, intestines, kidney  
60
HC
14-35 weeks (3): placenta, adrenal, thymus, brain  
60
HD
14-18 weeks  
60
HE
19-22 weeks  
60
HF
24-26 weeks  
60
HG
28-31 weeks  
60
HH
33-35 weeks  
60
Cell line array
XA
human gastric cancer cell lines  
32
XB
Human colorectal cancer cell lines  
58
XC
Human liver cancer cell lines  
20
XD
Human gastric cancer cell lines grown in nude mice  
34
XF
Human liver cancer cell lines grown in nude mice  
28
Animal tissue array
ZC
Normal organs of adult rats, formalin fixed  
23
ZD
Normal organs of adult rats, formalin fixed, 4.0mm  
23
ZE
Normal organs of adult mice, formalin fixed  
24
ZF
Normal organs of adult rats, perfusion fixation  
59
ZG
Normal organs of adult mice, perfusion fixation  
59
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