NoEndo JETSTAR ENDOTOXIN-FREE Plasmid Purification Kit

Rate this item
(1 Vote)
#222 010 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 10 preps   240 USD
#222 020 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 20 preps  393 USD
#222 100 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 100 preps  1457 USD
#232 006 NoEndo JETSTAR Endotoxin-Free Plasmid MEGAprep Kit / 6 preps  256 USD
#242 006 NoEndo JETSTAR Endotoxin-Free Plasmid GIGAprep Kit / 6 preps 584 USD
noendo maxi kit             noendo maxi saeule              noendo giga kartusche
                                                           NoEndo Maxi column      NoEndo Giga cartridge
Endotoxin content of purified DNA <0.001 EU/µg
TWO endotoxin removal steps instead of only 1
VERY QUICK processing times of vacuum-driven MEGA and GIGA cartridges
No additional incubation times in comparison to the JETSTAR standard protocol
Bacterial lysate remains CLEAR during endotoxin removal procedure
Short, convenient protocol
All components included in the kit
Buffers crucial for endotoxin removal tested and certified to contain <0.1 endotoxin units (EU) per ml
NoEndo JETSTAR specifications:
Product is designed to prepare ENDOTOXIN-FREE plasmid DNA from E. coli cells (maxi, mega and giga scale)
Endotoxin level of purified plasmid DNA is <0.001 EU/µg plasmid DNA
Product contents are gravity fast flow columns or ultra-quick vacuum cartridges, ready-to-use endotoxin removal and chromatography buffers, RNase A and protocol
No chromosomal DNA contamination
No phenol/chloroform extractions
No organic or toxic reagents
Excellent reproducibility
Total column procedure in 10 min (MEGA) – 45 min (Maxi)
Plasmid DNA is ultrapure (better than 2 x CsCl) with no known application limit
Plasmid DNA recovery is 90 – 95% on average
Plasmid DNA of all sizes can be prepared
Plasmid DNA yields are up to 750 µg (Maxi) / 2,500 µg (Mega) / 10,000 µg (Giga)
A260/280 ratio is > 1.85
Applications for plasmid and cosmid DNA
Transfection of primary cells (incl. neuronal cells)
Transfection of very endotoxin-sensitive eucaryotic cells
Microinjection into animals and eucaryotic cells
Gene therapy applications
Capillary sequencing
All fluorescent sequencing protocols
Manual sequencing
All standard cloning and enzymatic manipulations
Read 969 times