JETQUICK Plasmid Purification Spin Kit

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                                                                          Micro spin cup

 

 

#400 050 JETQUICK Plasmid Miniprep Spin Kit / 50        110 USD
#400 250 JETQUICK Plasmid Miniprep Spin Kit / 250      328 USD
#420 050 JETQUICK Gel Extraction Spin Kit / 50            117 USD
#420 250 JETQUICK Gel Extraction Spin Kit / 250          350 USD
#410 050 JETQUICK PCR Purification Spin Kit / 50         117 USD
#410 250 JETQUICK PCR Purification Spin Kit / 250       350 USD

 

Specifications:
 
Product is designed to prepare plasmid DNA (small scale) from E.coli cells
Product contents are micro spin cups, RNAse A, ready-to-use buffers and protocol
Very high DNA capacity (up to 50 µg plasmid DNA per micro-spin cup)
Ultra quick procedure using micro spin cups
No chromosomal DNA contamination
No ethanol or isopropanol precipitation
No phenol/chloroform extraction
No organic or toxic reagents
Excellent reproducibility
Culture volumes up to 5ml
Total procedure is < 25min
Plasmid sizes are up to 20.000bp
Plasmid recovery is 90% - 95% on average
Plasmid quality matches CsCl purified DNA
A260/280 ratio is > 1.8
DNA elution in TE buffer or equivalent low-salt buffer or simply water
Micro-spin cups are adaptable to vacuum manifold (see protocol links below)
 
Applications:
 
Fluorescent sequencing
Ligation
Restriction analysis
PCR template preparation
Labeling
All other enzymatic reactions

 

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A number of twelve plasmid preparations were carried out simultaneously from twelve separate 1 ml E.coli cultures using the JETQUICK Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose ge

 

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