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UNIFILTER® Filtration Microplates

UNIFILTER® Filtration Microplates
> UNIFILTER Microplate
The patented Whatman UNIFILTER microplates with filter-bottom wells are convenient and ready to use. Available in 24, 96 and 384 well formats, UNIFILTER microplates offer a choice of filter media to meet exact application requirements. 

The unique drip director design of Whatman UNIFILTER microplates ensures precise collection of the filtrate to allow for further processing and analysis. UNIFILTER microplates are available in a range of well volumes from 100 µL to 10 mL.

Features and Benefits

  • No crosstalk. Patented integral filter design prevents well-to-well cross contamination
  • Economical to use. Wide range of well volume options ensures efficient use of materials
  • Better control. Choice of filter media allows control of the flow rates and retention characteristics
  • Versatile. A broad range of filtration media is available including glass fibre, polypropylene, cellulose nitrate, cellulose acetate, nylon and ion exchange cellulose
Typical Data - UNIFILTER
Filter Media Flow Rate* Protein Binding Hydrophilic Solvent Resistance Physical Strength Thermal Resistance °C General Comments
Cellulose Nitrate (CN) 4 High Yes Poor Brittle <125 Highly adsorptive membrane (CN) typically used for DNA/RNA/ protein hybridization, also for ELISA and RIA-based assays.
Cellulose Acetate (CA) 3 Low Yes Poor Moderate <120 Typically used for low protein binding applications, good wet strength. General purpose microbiological filter.
Polypropylene (PP) 2 Negligible No Very Good Good <80 Typically used for prefiltration. Sensitive to gamma sterilization. Very low extractables, chemically inert.
Polyvinylidene fluoride (PVDF) Hydrophilic** 4 Low Yes Good Good <135 Low protein binding, good chemical resistance.
Glass Microfiber (GF) 5 Moderate Yes Very Good Poor High Wide range available. Typically used as absorptive or adsorptive wicking media and prefilters. Excellent particle retention and resistance to clogging. Used for DNA binding.
 
 
 

96 Well 800 µL UNIFILTER microplate

UNIFILTER® filtration microplates
> 96 Well 800 µL UNIFILTER microplate
96 Well 800 µL UNIFILTER microplate
The 800µL UNIFILTER is the microplate most typically used in purifications, isolations and separation of biomolecules, particularly DNA. 

The microplate has a well volume of 800µL which is ideal for standard DNA plasmid miniprep chemistries. The choice of short or long drip directors is application specific. The UNIFILTER 800µL is constructed from rigid high grade polystyrene.

96 Well 800 µL UNIFILTER® Microplate
Catalogue Number Well Format Well Volume (µL) Plate Material Drip Director Filter Media Quantity/Case
7700-1801 96 800 Clear Polystyrene Short Whatman GF/C 25
7700-1804 96 800 Clear Polystyrene Short 25–30 µm melt blown polypropylene 25
7700-1806 96 800 Clear Polystyrene Short 0.45 µm hydrophilic PVDF 25
7700-1808 96 800 Clear Polystyrene Short 0.45 µm Cellulose Acetate 25
7700-1818 96 800 Clear Polystyrene Short 5–7 µm melt blown polypropylene 25
7700-2801 96 800 Clear Polystyrene Long Whatman GF/C 25
7700-2803 96 800 Clear Polystyrene Long Whatman GF/B 25
7700-2804 96 800 Clear Polystyrene Long 25–30 µm melt blown polypropylene 25
7700-2805 96 800 Clear Polystyrene Long 0.45 µm PP membrane 25
7700-2806 96 800 Clear Polystyrene Long 0.45 µm hydrophilic PVDF 25
7700-2808 96 800 Clear Polystyrene Long 0.45 µm Cellulose Acetate 25
7700-2809 96 800 Clear Polystyrene Long 0.45 µm Nylon Positive 25
7700-2810 96 800 Clear Polystyrene Long DNA binding plate 25
7700-2811 96 800 Clear Polystyrene Long Whatman GF/D 25
7700-2817 96 800 Clear Polystyrene Long 10–12 µm melt blown polypropylene 25
7720-2830 96 800 Clear Polystyrene Long Lysate clarification plate 25
7700-2828 96 800 Clear Polystyrene Long Whatman oleophobic PKP 10
7770-0062 96 800 Clear Polystyrene Long 25 µm melt blown polypropylene over 0.45 µm PP membrane 25
 
  • Multiwell accessories
 
Multiwell accessories
> BugStopper microplate capmat
BugStopper™ microplate capmat
Whatman BugStopper™ Capmats provide a simple and reliable method for venting cultures being grown in a 24 well microplate. This reusable sterile closure, which is produced using chemically resistant biosafe silicone rubber, incorporates hydrophobic microfilters which provide an ideal vent for each well.
More efficient than plastic lids, test comparisons confirm that BugStopper Capmats improve cell growth and significantly reduce evaporation. The silicone rubber portion of the capmat reseals after puncture, thus keeping cell cultures sterile during inoculation or aspiration.

Sterile Venting Closures for Microplate Cultures

  • More efficient than plastic lids. Perfect for extended growth of slow growing bacteria and fungi
  • Positive seal for every well. Significantly reduces evaporation rate and eliminates well-to-well cross-contamination
  • Autoclave and re-use. Cost-effective; repeated autoclave cycles do not affect gas exchange or retention capabilities
  • Rated 99.9% efficient for bacteria and viruses. Restricts microorganisms while allowing H2O and CO2 to pass through the membrane
  • Prevents aerosol formation. Suitable for growth of infectious microorganisms
Ordering Information
Catalogue Number Well Format Item Material Quantity/Case
7704-0014 24 BugStopper Venting Capmat for 10 mL Microplates Silicone Rubber 5
7701-5102 24 Growth Plate, 10 mL, round-bottom Polypropylene 25
 
  • membrane filters

 

membrane filters

Membrane filters
 membrane filters
Whatman brings to the laboratory user a range of membrane filters whose advanced technical specifications makes them today's preferred choice for a wide range of applications. The membrane filters offer accurately controlled pore size distribution and higher strength and flexibility which ensure reproducibility and consistency. The Whatman membrane filter range includes pore sizes (from 0.02 to 5µm) with a wide selection of membrane filters. Sterile and autoclave packs are available for specialized applications. Colored and gridded types are also available.

To access the range of Whatman Membrane Filters, please click on the links on the navigation box to the right.

 

Anodisc inorganic membranes

Anopore® inorganic membranes
> Anodisc inorganic membranes
The Anopore® inorganic membrane is ideal for a wide range of laboratory filtration applications. This unique material has a precise, non-deformable honeycomb pore structure with no lateral crossovers between individual pores.that filters at precisely the stated cutoff, allowing no larger sized particles to pass through the membrane. The Anopore inorganic membrane is composed of a high purity alumina matrix that is manufactured electrochemically. The membrane also exhibits low protein binding, has minimal autofluorescence, is non-toxic and supports cellular growth. 

The precise pore structure and narrow pore size distribution of the Anopore membrane ensure a high level of particle removal efficiency. Micro-organisms and particulate material are captured on the surface of the membrane for subsequent analysis by light or electron microscopy. When wet, the membrane is virtually transparent, which means that retained particles do not need to be transferred to another surface before microscopic examination.

> Anodisc pore structure

Anopore® inorganic membranes
> Anodisc pore structure

The membrane is hydrophilic and is compatible with most solvents and aqueous material. No monomers, plasticizers, adhesives, surfactants or wetting agents are used in the manufacturing process, which eliminates sample contamination and ensures low protein binding and minimal loss of sample.

The Anopore membrane is supplied in the form of Anodisc membrane filters. The membrane is peripherally bonded to an annular polypropylene ring (except the 13 mm diameter disc) for ease of handling and is suitable for both vacuum and pressure filtration.

Anopore is available in 3 nominal pore sizes: 0.02µm, 0.1µm and 0.2µm and in 3 disposable syringe filter housings: 13mm, 25mm and 47mm.

Features and Benefits

  • High pore density and narrow pore size distribution make it an extremely precise membrane
  • Wide solvent compatibility reduces the need to stock a variety of membranes in the laboratory
  • No additives used in the manufacturing process ensures minimal extractables and no sample contamination
  • Extremely low protein binding minimizes sample loss
  • Virtually transparent when wet making it ideal for microscopy studies

Applications

  • HPLC mobile phase filtration and degassing
  • Ultra cleaning of solvents
  • Gravimetric analysis
  • Liposome extrusion
  • Scanning electron microscopy studies
  • Bacterial analysis by epifluorescence light microscopy
  • Micrometer and nanometer filtration
  • Metal nanorods formation
Typical Data
  Anodisc 13 Anodisc 25 Anodisc 47
Average Membrane Thickness 60 µm 60 µm 60 µm
Membrane Diameter 13 mm 21 mm 43 mm
Membrane Type Anopore    
  aluminum oxide    
Support Ring Material None Polypropylene Polypropylene
Construction Process None Thermal weld Thermal weld
Protein Adsorption Low Low Low
Burst Strength 65–110 psi 65–110 psi 65–110 psi
Maximum Service Temperature 400°C 400°C 400°C
Porosity 25-50% 25-50% 25-50%
Autoclavable Yes No No
Refractive Index 1.6 1.6 1.6
Ordering Information - Diameter (mm)
Diameter (mm) Pore Size (µm) Membrane Catalogue Number Hydrophilic Protein Binding Solvent Resistance Quantity/Pack
13 Anodisc 13* 0.02 6809-7003 Yes Low Very Good 100
13 Anodisc 13* 0.1 6809-7013 Yes Low Very Good 100
13 Anodisc 13* 0.2 6809-7023 Yes Low Very Good 100
25 Anodisc 25 0.02 6809-6002 Yes Low Very Good 50
25 Anodisc 25 0.1 6809-6012 Yes Low Very Good 50
25 Anodisc 25 0.2 6809-6022 Yes Low Very Good 50
47 Anodisc 47 0.02 6809-5002 Yes Low Very Good 50
47 Anodisc 47 0.1 6809-5012 Yes Low Very Good 50
47 Anodisc 47 0.2 6809-5022 Yes Low Very Good 50
 
 

  • Application specific plates
     

 

Application specific plates
> ELISA UNIFILTER
ELISA UNIFILTER®
The Whatman ELISA plate allows researchers to use the binding characteristics of nitrocellulose in the standard SBS 8 x 12 multiwell format. It is also suitable for other types of ligand binding applications such as hybridoma supernatant screening. Solutions are easily vacuumed to waste using a vacuum manifold.
Chemiluminescent assays can be read in the plate. If the ELISA product is eluted then centrifugation is preferred. This microplate is compatible with automated systems.
Ordering Information - ELISA UNIFILTER®
Catalogue Number Well Format Well Volume Plate Material Filter Media Well Bottom Quantity/Case
7700-3307 96 350 µL White Polystyrene 0.45µm CN * Filter 50
7701-1350 96 300 µL Clear Polystyrene N/A Flat 50
7701-5200 96 2 mL Natural Polypropylene N/A Round 25
7705-0107 UniVac™ Vacuum to Collect Manifold, acrylic 1
7704-0001 Clear Polyester ThinCold Sealing Film Adhesive Backing 100
* CN = Cellulose nitrate
 
  • Protein precipitation UNIFILTER FF

 
Application specific plates
> Protein precipitation UNIFILTER FF
 

Protein precipitation UNIFILTER® FF

The Whatman Protein Precipitation UNIFILTER FF (Fast Flow)is optimized for removing acetonitrile-precipitated proteins from plasma or serum samples. Made with 2 mL, 96 well, rigid glass-filled polypropylene microplates, the Whatman Protein Precipitation UNIFILTER is both robust and chemically resistant.
 

The plates contain specially formulated dual membranes with two distinct layers. The top layer acts as a prefilter to remove coarse particulates. The bottom layer is oleophobic for retaining the well contents without dripping. This provides a final filter for removing fine particulate matter when a vacuum is applied. The plates are available in the following well bottom formats: Standard; Fast Flow; Round Bottom and "V" Bottom.

Features and Benefits
 
  • 96 samples purified at the same time
  • Purified samples available in less than 10 minutes
  • Precipitation and filtration in the same well
  • No laborious pipetting and/or centrifugation required and minimal liquid handling
  • Dual Whatman filter media
  • Ensures no fluid loss during incubation and fast flow during filtration
  • Over 98% protein removal
  • Sample volumes up to 150µL for plasma and 200µL for serum
  • SBS compatible with the exception of the "V" bottom plate
  • Robotics friendly
Ordering Information - Protein Precipitation UNIFILTER® FF
Catalogue Number Well Format Well Volume Plate Material Well Bottom Quantity/Case
7720-7235 96 2 mL Glass Polypropylene Standard 1
7720-7236 96 2 mL Glass Polypropylene Fast Flow 5
7701-5750 96 750 µL Natural Polypropylene Round Bottom 25
7701-5200 96 2 mL Natural Polypropylene Round Bottom 25
 
 
Application specific plates
> 384 Well DNA binding UNIFILTER
384 Well DNA binding UNIFILTER®
The 384 Well DNA Binding UNIFILTER plate effectively binds and purifies DNA molecules. It provides highly reproducible results with yields exceeding 2 µg/well, from bind-wash-elute processing with collection by filtration. Minimal liquid hang up allows for reduced elution volume, enabling DNA concentration as high as 150 ng/µL. Further ethanol precipitation is unnecessary. The DNA is ready to use.
 

Features and Benefits

  • High efficiency bind-wash-elute processing with collection by filtration
  • simplifies automation with no cross contamination
  • Highly reproducible results yielding DNA purity exceeding 2µg per well sufficient for sensitive downstream applications
  • Minimal liquid hang up allows for reduced elution volume with DNA concentration as high as 150ng/µL
Ordering Information - 384 Well DNA Binding UNIFILTER®
Catalogue Number Well Format Well Volume (µL) Plate Material Filter Media Quantity/Case
7700-2110 384 100 Clear Polystyrene DNA Binding 50
7701-1100 384 100 Clear Polystyrene N/A 50

Flexible capmats
 
Multiwell accessories
> Flexible capmats
Flexible capmats
Whatman flexible capmats individually seal the top of each well. Capmats may be used on either filter or collection microplates. 
 
Ordering Information
Capmats
Catalogue Number Well Format Capmat Material Microplate Compatibility Quantity/Case
7704-0004 96 Square Format EVA 2 mL microplates 100
7704-0005 96 Round Format EVA 750 µL and 800 µL microplates 100
7704-0006 48 Rectangular Format EVA 5 mL microplates 100
7704-0007 24 Square Format Santoprene 10 mL microplates 100
7704-0015 384 Square Format Santoprene 400 µL microplate 100
Pierceable Capmats
7704-0104 96 Square Format Silicone 2 mL microplates 50
7704-0105 96 Round Format Silicone 300 µL, 750 µL and 800 µL microplates 50
7704-0115 384 Square Format Silicone 100 µL and 400 µL microplates 50
Venting Capmats (autoclavable)
7704-0014 24 BugStopper Venting 10 mL microplates 5
 
 
 
 
 

 Chromatography cellulose media

Chromatography cellulose media
> Chromatography cellulose media
Whatman offers an extensive product line of chromatography cellulose media for applications ranging from the separation of biopolymers such as proteins, peptide and hormones to the purification of monoclonal antibodies, vaccines, synthetic pharmaceuticals and agrochemicals. 

Technological innovations in product development and manufacturing processes and a dedication to excellence are combined to ensure batch-to batch reproducibility of these chromatography cellulose media.

 
 

Bulk silica media for column chromatography

Bulk silica media for column chromatography
> Bulk silica media for column chromatography
The Whatman bulk silica media is used for low pressure columns. Same base silica and chemistries as those used for Whatman TLC plates. Particularly useful for scaling up separations optimized by TLC. In addition, Whatman offers an all-purpose media for general column chromatography. Applicable for preparative low pressure column separations scaled up from Partisil analytical HPLC columns.
 

Features and Benefits

  • High resolution
  • Good flow characteristics
  • High surface area
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for Low Pressure Column Chromatography
4771-010 LPS-2 low pressure silica gel 37–53 Adsorption 450 m2/g; may be dry packed 1 kg
4776-001 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 100 g
4776-005 LRP-2 ODS bonded silica gel 37–53 Reversed phase LPS-2 silica bonded with 16% C; - may be dry packed; end-capped 500 g

 
Ordering Information
Catalogue Number Product Particle Size (µm) Other Specifications Weight
Bulk Silica Media for General Column Chromatography
4132-100 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 100 g
4132-301 ODS-3 (C-18) 32-75 Reversed Phase Octadecyl-bonded; end-capped; - polymeric; 10.5% carbon load 1 kg
4790-010 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 1 kg
4790-050 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 5 kg
4790-250 Silica, 60Å 230-400 mesh - (38-63) Adsorption Bulk media for column or - dry chromatography 25 kg
4791-005 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 500 g
4791-010 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 1 kg
4791-050 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 5 kg
4791-250 Silica, 60Å 70-230 mesh - (63-212) Adsorption Bulk media for column or - dry chromatography 25 kg

NEW Purasil 60Å Silica Gel

Silica Gel Media for Flash Chromatography 
Whatman Purasil high purity silica gel provides an excellent separation medium for flash chromatography purification of target molecules. Narrow particle size distribution and minimal fines enable fast separations with no loss of chromatographic performance.

Features and Benefits

  • High resolution
  • Excellent flow rates
  • Direct scalability
Typical Data
Iron Content <0.02%
Chloride Content <0.10%
Loss on Drying <7%
pH (10% suspension) 7 ± 0.5
Pore Volume 60–80 mL/g
Surface Area 500–600 m2/g
Ordering Information
Catalogue Number Product Dimensions
4745-010 230–400 mesh (38–63 µm) 1 kg
4745-250 230–400 mesh (38–63 µm) 25 kg
4746-010 70–230 mesh (63–210 µm) 1 kg
4746-250 70–230 mesh (63–210 µm) 25 kg
 
 
 

Collection plates

Collection plates
> Collection plates
Whatman microplates for collection and analysis are available in 24, 48, 96 and 384 well formats, all unique to Whatman. These microplates are manufactured from polystyrene, polypropylene and Multi-Chem™ materials to accommodate a wide range of sampling and storage applications. 

To access the range of Whatman Collection Plates, please click on the links on the navigation box to the right.

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