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|GenSpin™ Genomic DNA Purification Kit|
> GenSpin genomic and plant DNA purification kit
GenSpin™ Genomic DNA Purification Kit
This simple protocol uses a single micro centrifuge tube and small sample volume (5-50 µL) to produce high quality DNA for amplification by PCR. DNA for up to 80 amplification reactions can be obtained from a 50 µL fresh or anticoagulant-treated blood sample.
GenSpin incorporates patented FTA technology, which lyses cell and nuclear membranes on contact. The DNA is reversibly entrapped within this type of FTA filter matrix and can be stored for weeks at room temperature prior to purification. Cellular debris and proteins are removed by washing with GenSpin Buffer and TE-1 Buffer using a centrifuge. The purified DNA is released from this specialized filter matrix by heat elution and is ready for immediate PCR amplification.
Features and Benefits
*Extraction of these plant species requires the addition of DTT to Homogenization Buffer.
DNA yields can vary depending on plant species, tissue age and growing conditions.
Double-stranded DNA was quantified using PicoGreen™ Reagent
* Manual method (Dellaporta et. al. 1983), does not include time required for full resuspension after DNA precipitation.
Reference: Dellaporta et. al. (1983) A plant DNA Minipreparation. Plant Molecular Biology Reporter 1:19-21
GenSpin™ Plant DNA Purification Kit
The GenSpin Plant DNA Purification Kit is designed for the rapid preparation of double-stranded DNA in solution from small quantities of plant material for PCR analysis. Using a single microcentrifuge tube, this simple protocol enables the recovery of DNA for more than 50 amplification reactions from just 10 mg of plant tissue. The small sample capability is ideally suited for rapid analysis studies such as identification of genetically-modified plants and cultivar screening.
Plant material is homogenized at room temperature and applied to the GenSpin Filter Basket, which incorporates FTA technology to immediately stabilize the DNA at room temperature. Nucleases are inactivated and the DNA is protected from UV and environmental damage. The immobilized DNA is entrapped in the fibres of the matrix and can either be purified immediately or stored at room temperature for more than 4 weeks. The filter is washed with two reagents to remove contaminants that would inhibit PCR. The DNA is then eluted from the filter by heating and collected by centrifugation.
Features and Benefits