Nucleic Acid

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Collection, storage and purification
 
 
Collection, storage and purification
> Whatman FTA cards
FTA® Technology 
Collect, Transport, Archive and Isolate Nucleic Acids - All at Room Temperature!
FTA Cards utilize patented Whatman FTA Technology that simplifies the handling and processing of nucleic acids.
 

FTA Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidation and UV damage. FTA Cards rapidly inactivate organisms, including blood-borne pathogens, and prevent the growth of bacteria and other microorganisms. Try FTA, and you'll soon find it's an indispensable part of your DNA toolbox. US Patent Nos. 5496562, 5756126, 5807527, 5972386, 5985327 and other patents pending

Features and Benefits

Collection, storage and purification

Electron micrograph showing DNA entrapped within the FTA matrix (magnification x 10,000).

Capture nucleic acid in one easy step

  • Captured nucleic acid is ready for downstream applications in less than 30 minutes
  • Nucleic acids collected on FTA Cards are stable for years at room temperature
  • FTA Cards are stored at room temperature before and after sample application, reducing the need for laboratory freezers
  • Suitable for virtually any cell type
  • Indicating FTA Cards change colour upon sample application to facilitate handling of colourless samples
  • FTA Cards are available in a variety of configurations to meet application requirements
  • Custom configurations are available on request

pplications

  • Forensics
  • Transgenics
  • Transfusion Medicine
  • Plasmid Screening
  • Food & Agriculture Testing
  • Drug Discovery
  • Genomics
  • STR Analysis
  • Animal Identification
  • Diagnostics
  • Pharmacogenomics
  • Molecular Biology

Capture Nucleic Acids in One Easy Step

Simply apply your sample to the FTA Card. Cell membranes and organelles are lysed and the released nucleic acids are entrapped in the fibres of the card. The nucleic acids remain immobilized and are stabilized for transport, immediate processing or long-term room temperature storage.

Since captured nucleic acids are stabilized, FTA Cards facilitate sample collection in remote locations and simplify sample transport. For example, you can collect samples deep in a rain forest without worrying about immediate refrigeration. Ship your samples back to the lab without expensive special handling or dry ice, and process at your convenience.

Indicating FTA Cards are recommended for colourless samples. These FTA Cards change from pink to white when sample is applied, verifying the location of the sample.

FTA Cards Used With Virtually Any Sample Type

  • Blood
  • Cultured Cells
  • Buccal Cells
  • Plant Material
  • Bacteria
  • Plasmids
  • Microorganisms
  • Solid Tissue
  • Viral Particles
  • M13 Plaques
  • ...and more

Captured Nucleic Acid is Ready for Downstream Applications in Less than 30 Minutes
Captured nucleic acids are ready for purification when you are. Just take a punch from the FTA Card, wash with FTA Purification Reagent and rinse with TE-1 buffer. DNA on the washed punch is ready to use in applications such as PCR, RFLP analysis and RT-PCR. Since PCR products remain in solution, the punch can be used for multiple amplifications.

Store Nucleic Acids at Room Temperature for Years
Genomic DNA stored on FTA Cards at room temperature for over 14 years (and counting) has been successfully amplified by PCR. In contrast, genomic DNA stored at room temperature on non-FTA Cards for over six months did not amplify.

Sample integrity is optimized when FTA Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.

FTA Cards offer a compact room-temperature storage system that reduces the need for precious freezer space.

FTA Classic Card 
Four sample areas for application of up to 500 µL whole blood or 100 µL plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples on one card. Different samples can be processed independently.

Indicating FTA Classic Card 
Same as FTA Classic Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with colourless samples such as buccal or culture cells.

FTA Mini Card 
Two sample areas for application of up to 250 µL whole blood or 50 µL plant homogenate per card. Convenient for protocols that require different locations for testing and archiving samples. Different samples can be processed independently.

Indicating FTA Mini Card 
Same as FTA Mini Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Micro Card 
One sample area for application of up to 125 µL whole blood or 25 µL plant homogenate per card. Recommended when only one sample is needed.

Indicating FTA Micro Card 
Same as FTA Micro Card with a colour indicator that changes from pink to white when sample is applied. Recommended for use with clear samples such as buccal or culture cells.

FTA Gene Card 
An FTA card enclosed in a rigid card frame. Three sample areas for application of up to 225 µL whole blood or 30 µL plant homogenate per card. Can be utilized in many automatic dispensing/pipetting systems when used with the FTA Gene Card Tray (WB100030).

PlantSaver™ FTA Card 
Plant friendly FTA Card, in a Classic Card format. Features a laminated flap that allows you to vigorously pound the plant sample into the FTA matrix without damaging the FTA Card.

FTA Kit 
Includes: 25 FTA Micro Cards, 2 vials purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

FTA Starter Pack 
Includes: 1 FTA Classic Card, 1 FTA Mini Card, 1 FTA Micro Card, 1 Indicating FTA Mini Card, 1 FTA Indicating Micro Card, 2 foam-tipped applicator swabs, 1 multi-barrier pouch and desiccant, 1 vial purification reagent (25 mL), 2 Harris Uni-Core Punches with cutting mat, instructions.

Blood Stain Card 
Contains non-FTA absorbent filter paper for the collection and transport of blood and body fluids. Intended for short-term handling of specimens and for protein or metabolite studies. For long-term storage of nucleic acids, Whatman FTA Cards are recommended. There are four sample areas for application of up to 500 µL whole blood per card. Convenient for multiple applications of the same specimens or collection of multiple specimens on one card. Different samples can be processed independently.

Ordering Information - Whatman FTA ®Technology - Nucleic Acid Collection, Storage and Purification
Catalogue Number Description Cards/
Pack
Sample Areas/
Card
Maximum Volume/
Sample Area (µL)
Maximum Total
Volume/Card (µL)
WB120067 FTA Kit 25
WB120061 FTA Starter Pack N/A
WB120305 FTA Classic Card 25 4 125 500
WB120205 FTA Classic Card 100 4 125 500
WB120306 Indicating FTA Classic Card 25 4 125 500
WB120206 Indicating FTA Classic Card 100 4 125 500
WB120355 FTA Mini Card 25 2 125 250
WB120055 FTA Mini Card 100 2 125 250
WB120356 Indicating FTA Mini Card 25 2 125 250
WB120056 Indicating FTA Mini Card 100 2 125 250
WB120310 FTA Micro Card 25 1 125 125
WB120210 FTA Micro Card 100 1 125 125
WB120311 Indicating FTA Micro Card 25 1 125 125
WB120211 Indicating FTA Micro Card 100 1 125 125
WB120208 FTA Gene Card 25 3 75 225
WB120208 FTA Gene Card 100 3 75 225
WB120365 PlantSaver FTA Card 25 4
WB120065 PlantSaver FTA Card 100 4
WB100014 Blood Stain Card, non-FTA 100 4 125 500
WB120217 FTA Card/Pouch/Desicant 1000
WB120216 FTA Gene Card/Pouch/Desiccant 1000
 

For Collection, Storage, Processing an Shipping FTA Cards

FTA® reagent and accessories
FTA Purification Reagent
  • For purification of nucleic acids stored on FTA Cards
  • Ensures superior quality DNA for PCR or RFLP analysis
  • Removes heme, PCR inhibitors and other potential contaminants
  • Non-toxic, hypoallergenic aqueous solution

FTA Gene Card Tray

  • Holds 2 FTA Gene Cards for use in automatic dispensing/pipetting systems
  • Tray footprint conforms to SBS standards

 Harris Micro Punches

FTA® reagent and accessories
> Harris Micro Punches

Harris Micro Punches (1.2 mm or 2.0 mm) and Cutting Mat

  • Recommended for the precise punching of FTA Cards. No sample carryover when recommended procedures are used. Tips provide up to 2000 punches. Polished steel tip is case hardened and can be sterilized. The cutting mat ensures clean sample cuts and extends the life of the cutting tip
  • 1.2 mm punch recommended for use with FTA Cards containing whole blood and samples with high DNA content
  • 2.0 mm punch recommended for use with FTA Cards containing buccal cells, plasmids and other samples with lower DNA content

Sterile Foam Tipped Applicator

  • For the collection of saliva and buccal cells
  • Non-abrasive foam head is same size as sample area on Indicating FTA Cards to facilitate sample application

Sterile Omni Swab

This is a non-invasive device for the collection of saliva and cheek buccal cells. Sterile Omni Swab features a unique brush-like swab head that easily ejects from the stem of the swab for transfer of samples into tubes and multiwell plates. Sterile Omni Swabs are pre-sterilized and individually wrapped for single use.

 Multi-Barrier Pouches

FTA® reagent and accessories
> Multi-Barrier Pouches

Multi-Barrier Pouches

Large

  • For transporting or storing FTA Classic Cards
  • Seven laminated layers that protect the card from exposure to gas or liquid contamination
  • Tamper-evident seal maintains sample security
  • Outer paper surface for labelling or writing

Small

  • Same construction in a smaller size for storing FTA Gene Cards, Mini Cards or Micro Cards

FTA Card Mailer

  • For use with the Multi-Barrier Pouches to transport FTA Cards in accordance with US Postal Service requirements

Storage Desiccant Packets

  • Ensure that FTA Cards remain dry during transport or storage
  • Changes from blue to pink to indicate absorption of moisture
Ordering Information - FTA Reagent and Accessories
Catalogue Number Description Quantity/Case
WB120204 FTA Purification Reagent 500 mL
WB100030 FTA Gene Card Tray 20
WB100032 Sterile Foam Tipped Applicators 100
WB100005 Harris Micro Punch 1.2 mm (with Mat) 1
WB100006 Replacement Tip 1.2 mm 1
WB100007 Harris Micro Punch 2.0 mm (with Mat) 1
WB100008 Replacement Tip 2.0 mm 1
WB100020 Replacement Cutting Mat 1
WB100010 Multi-Barrier Pouch, Large (9 x 15 cm) 500
WB100011 Multi-Barrier Pouch, Small (8 x 7 cm) 500
WB100016 FTA Card Mailer 50
WB100003 Desiccant Packets (1 gm) 1000
WB100021 Bloodstain Card/Pouch/Desiccant 500
WB100025 Harris Micro Punch 1.2 mm replacement plunger 1
WB100026 Harris Micro Punch 2.0 mm replacement plunger 1
WB100028 Harris Uni-Core 1.2 mm punch 4
WB100029 Harris Uni-Core 2.0 mm punch 4
WB100035 Sterile Omni Swab 100
 
GenSpin™ Genomic DNA Purification Kit
 

GenSpin™ genomic and plant DNA purification kits> GenSpin genomic and plant DNA purification kit

GenSpin™ Genomic DNA Purification Kit 
The GenSpin Purification Kit is designed to purify high quality, PCR-ready, single stranded DNA in solution from whole blood and cultured cells in as little as 25 minutes.

This simple protocol uses a single micro centrifuge tube and small sample volume (5-50 µL) to produce high quality DNA for amplification by PCR. DNA for up to 80 amplification reactions can be obtained from a 50 µL fresh or anticoagulant-treated blood sample.

GenSpin incorporates patented FTA technology, which lyses cell and nuclear membranes on contact. The DNA is reversibly entrapped within this type of FTA filter matrix and can be stored for weeks at room temperature prior to purification. Cellular debris and proteins are removed by washing with GenSpin Buffer and TE-1 Buffer using a centrifuge. The purified DNA is released from this specialized filter matrix by heat elution and is ready for immediate PCR amplification.

Features and Benefits

  • Simple, single tube protocol. Purify single stranded DNA from whole blood and cultured cells in less than 25 minutes
  • FTA technology lyses cells, denatures proteins and inactivates viral contaminants. Allows room temperature storage of DNA and safe sample transport and handling prior to purification
  • High quality DNA. Enables full length PCR amplification
  • Highly efficient method. Enables purification of sufficient DNA for up to 80 amplification reactions from a 50 µL blood sample
  • No precipitation steps. DNA is ready for immediate analysis
GenSpin Genomic DNA Purification Kit Contents
Quantity Item
50 DNA Isolation Filter Baskets in 2 mL microcentrifuge tubes
50 2 mL microcentrifuge tubes
1 GenSpin Buffer 110 mL
1 Nuclease Free Water 25 mL
1 User Manual
Ordering Information - GenSpin Genomic DNA Purification Kits
Catalogue Number Description Size
WB120005 GenSpin DNA Purification Kit 50 purifications
WB120111 GenSpin Sample Kit 5 purifications
GenSpin Plant DNA Purification Kit Contents
Quantity Item
50 GenSpin Purification Tube with Filter Basket
50 GenSpin Collection Tube
1 bottle Homogenization Buffer 25 mL
1 bottle Wash Reagent 60 mL
1 bottle Rinse Reagent 60 mL
1 Instruction Booklet
Ordering Information - GenSpin Plant DNA Purification Kits
Catalogue Number Description Size
WB120046 GenSpin Plant Kit 50 Purifications
SWB120046 GenSpin Plant Sample Kit 5 Purifications
Table 1. Plant Species - Typical DNA Yields from 10 mg of Young Leaf Tissue
Plant Species Double Stranded DNA Yield (ng)
Alfalfa 800
Arabidopsis thaliana 110
Barley* 670
Brassica sp. 800
Corn* 120
Cotton 450
Potato 2200
Rice 120
Ryegrass 340
Soybean* 500
Spinach 340
Tobacco 1100
Tomato 1800
Wheat* 710

*Extraction of these plant species requires the addition of DTT to Homogenization Buffer. 
DNA yields can vary depending on plant species, tissue age and growing conditions. 
Double-stranded DNA was quantified using PicoGreen™ Reagent
Table 2. Comparison of GenSpin Plant and a Common Manual DNA Isolation Method
Extraction Time GenSpin Plant 25 minutes Manual Method* 90 minutes*
Homogenization Room Temperature Liquid Nitrogen
Precipitation/Resuspension Not Required Required
All Reagents Aqueous Yes No
Archiving Capability Yes No
PCR of Low-Copy Loci Yes Yes
Double-Stranded DNA Yes Yes
gDNA Isolation from Other Cell Types (bacteria, blood) Yes No
Pathogen Inactivation Yes No

* Manual method (Dellaporta et. al. 1983), does not include time required for full resuspension after DNA precipitation. 
Reference: Dellaporta et. al. (1983) A plant DNA Minipreparation. Plant Molecular Biology Reporter 1:19-21

GenSpin™ Plant DNA Purification Kit

The GenSpin Plant DNA Purification Kit is designed for the rapid preparation of double-stranded DNA in solution from small quantities of plant material for PCR analysis. Using a single microcentrifuge tube, this simple protocol enables the recovery of DNA for more than 50 amplification reactions from just 10 mg of plant tissue. The small sample capability is ideally suited for rapid analysis studies such as identification of genetically-modified plants and cultivar screening.

Plant material is homogenized at room temperature and applied to the GenSpin Filter Basket, which incorporates FTA technology to immediately stabilize the DNA at room temperature. Nucleases are inactivated and the DNA is protected from UV and environmental damage. The immobilized DNA is entrapped in the fibres of the matrix and can either be purified immediately or stored at room temperature for more than 4 weeks. The filter is washed with two reagents to remove contaminants that would inhibit PCR. The DNA is then eluted from the filter by heating and collected by centrifugation.

Features and Benefits

  • Simple, single tube protocol. Eliminates need for organic solvents, liquid nitrogen and time-consuming precipitation steps
  • Fast purification of DNA. Purify DNA in less than 30 minutes for quick sample screening. Up to 50 amplifications from only 10 mg of plant material
  • PCR-ready double-stranded DNA. Reliable amplification of DNA for a wide range of applications including cultivar screening and ID of genetically modified plants
  • FTA technology protects DNA from degradation. Enables room temperature storage for weeks
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