Micro spin cup
#400 050 JETQUICK Plasmid Miniprep Spin Kit / 50 110 USD |
#400 250 JETQUICK Plasmid Miniprep Spin Kit / 250 328 USD |
#420 050 JETQUICK Gel Extraction Spin Kit / 50 117 USD |
#420 250 JETQUICK Gel Extraction Spin Kit / 250 350 USD |
#410 050 JETQUICK PCR Purification Spin Kit / 50 117 USD |
#410 250 JETQUICK PCR Purification Spin Kit / 250 350 USD |
Specifications: | |
Product is designed to prepare plasmid DNA (small scale) from E.coli cells | |
Product contents are micro spin cups, RNAse A, ready-to-use buffers and protocol | |
Very high DNA capacity (up to 50 µg plasmid DNA per micro-spin cup) | |
Ultra quick procedure using micro spin cups | |
No chromosomal DNA contamination | |
No ethanol or isopropanol precipitation | |
No phenol/chloroform extraction | |
No organic or toxic reagents | |
Excellent reproducibility | |
Culture volumes up to 5ml | |
Total procedure is < 25min | |
Plasmid sizes are up to 20.000bp | |
Plasmid recovery is 90% - 95% on average | |
Plasmid quality matches CsCl purified DNA | |
A260/280 ratio is > 1.8 | |
DNA elution in TE buffer or equivalent low-salt buffer or simply water | |
Micro-spin cups are adaptable to vacuum manifold (see protocol links below) | |
Applications: | |
Fluorescent sequencing | |
Ligation | |
Restriction analysis | |
PCR template preparation | |
Labeling | |
All other enzymatic reactions |
A number of twelve plasmid preparations were carried out simultaneously from twelve separate 1 ml E.coli cultures using the JETQUICK Plasmid Miniprep Kit. The plasmid was pT7T3 19U. Aliquots were run on a 1% agarose ge
#200 050 JETSTAR 2.0 Plasmid Miniprep Kit / 50 194 USD |
#200 050 JETSTAR 2.0 Plasmid Mini columns / 50 194 USD |
#200 200colJETSTAR 2.0 Plasmid Mini columns / 200 517 USD |
#2002 00 JETSTAR 2.0 Plasmid Miniprep Kit / 200 538 USD |
#210 025 JETSTAR 2.0 Plasmid Midiprep Kit / 25 175 USD |
#210 050 JETSTAR 2.0 Plasmid Midiprep Kit / 50 278 USD |
# 210 250 JETSTAR 2.0 Plasmid Midiprep Kit / 250 1004 USD |
#220 010 JETSTAR 2.0 Plasmid Maxiprep Kit / 10 175 USD |
#220 020 JETSTAR 2.0 Plasmid Maxiprep Kit / 20 278 USD |
#220 100 JETSTAR 2.0 Plasmid Maxiprep Kit / 100 1072 USD |
#230 006 JETSTAR 2.0 Plasmid MEGAprep Kit / 6 185 USD |
#230 012 JETSTAR 2.0 Plasmid MEGAprep Kit / 12 310 USD |
#230 024 JETSTAR 2.0 Plasmid MEGAprep Kit / 24 550 USD |
#240 006 JETSTAR 2.0 Plasmid GIGAprep Kit / 6 352 USD |
#240 012 JETSTAR 2.0 Plasmid GIGAprep Kit / 12 600 USD |
JETSTAR specifications: | |
Product is designed to prepare plasmid DNA (small, medium and large scale) fromE.coli cells | |
Product contents are gravity fast flow columns or vacuum cartridges, RNAse A, ready-to-use buffers and protocol | |
JETSTAR preparations are available in Mini, Midi, Maxi, Mega and Giga format | |
JETSTAR is able to prepare cosmid DNA and BAC DNA as well | |
No chromosomal DNA contamination | |
No phenol/chloroform extraction | |
No organic or toxic reagents | |
Excellent reproducibility | |
Total procedure is 40 - 60min | |
Plasmid DNA is ultrapure (better than 2 x CsCl) | |
Plasmid DNA recovery is 90% - 95% on average | |
Plasmid DNA of all sizes can be prepared | |
Plasmid DNA yields are 20µg (Mini), 100µg (Midi), 500µg (Maxi), 2.500µg (Mega), 10.000µg (Giga) in minimum | |
A260/280 ratio is > 1.85 | |
Endotoxin level is < 0.01 EU/µg plasmid DNA | |
Applications for plasmid and cosmid DNA | |
Transfection | |
Manual and automated sequencing, including capillary sequencing | |
Microinjection | |
In vitro transcription | |
Restriction analysis, ligation, labeling etc. | |
Transfection of endotoxin-sensitive cells (Endo Free) | |
Gene therapy applications |
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LFU-Unit Midi column Maxi column
#211 025 JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25 256 USD |
#211 100 JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100 761 USD |
#221 020 JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20 401 USD |
#221 100 JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 100 1484 USD |
Advantages: | |
Very convenient procedure - much simpler and shorter than any competitor's protocol | |
Great time-saving compared to standard procedure | |
Newly designed FAST Midi columns much faster than any comparable product | |
Larger culture volumes can be processed on the new FAST Midi columns | |
No centrifugation necessary to clear bacterial lysate | |
Lysate clarification integrated into column procedure | |
Only ONE column wash for the new FAST Midi and Maxi columns | |
No clogging of the lysate filter unit | |
Much less susceptible against overloading than competitor's products | |
JETSTAR specifications: | |
Product is designed to prepare plasmid DNA (medium and large scale) from E.colicells | |
Product contents are gravity fast flow columns, RNase A, ready-to-use buffers and protocol | |
JETSTAR / LFU preparations are available in Midi and Maxi format | |
JETSTAR is able to prepare cosmid DNA and BAC DNA as well | |
No chromosomal DNA contamination | |
No phenol/chloroform extraction | |
No organic or toxic reagents | |
Excellent reproducibility | |
Total procedure in 30 - 40 min | |
Plasmid DNA is ultrapure (better than 2 x CsCl) | |
Plasmid DNA recovery is 90% - 95% on average | |
Plasmid DNA of all sizes can be prepared | |
Plasmid DNA yields are up to 200 µg (Midi) and up to 850 µg (Maxi) | |
A260/280 ratio is > 1.85 | |
Endotoxin level is < 0.01 EU/µg plasmid DNA | |
Applications for plasmid and cosmid DNA | |
Transfection | |
Manual and automated sequencing, including capillary sequencing | |
Microinjection | |
In vitro transcription | |
Restriction analysis, ligation, labeling etc. | |
Transfection of endotoxin-sensitive cells (Endo Free) | |
Gene therapy applications |
#222 010 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 10 preps 240 USD |
#222 020 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 20 preps 393 USD |
#222 100 NoEndo JETSTAR Endotoxin-Free Plasmid Maxiprep Kit / 100 preps 1457 USD |
#232 006 NoEndo JETSTAR Endotoxin-Free Plasmid MEGAprep Kit / 6 preps 256 USD |
#242 006 NoEndo JETSTAR Endotoxin-Free Plasmid GIGAprep Kit / 6 preps 584 USD |
ExiProgen is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run.
DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein.
The crude protein extract is further purified, using His-Tag engineered into the proteins.
The net result is up to 100 ug of >90% pure Protein in about 6 hours.
ExiProgen can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.
Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples
ExiProgen has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols
Cooling Block
ExiProgen has a built-in cooling block where the elution tube rack sits.
Sample integrity is ensured by keeping the samples below 10°C.
This allows for overnight runs and provides you with confidence in your results.
Magnetic block & Heating block
To increase extraction efficiency, the ExiProgen has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.
Contamination Shield
ExiProgen comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.
Easy to use LCD Touch screen
The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.
UV lamp
ExiProgen has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.
ExiProgen Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure.
ExiProgen DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA.
Price: 20 250 USD