Anti-Hu TdT Purified

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.

RUO

Mouse

Human

His-TdT recombinant protein

TdT

IgG2a

41A

Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

FC, WB, IHC-P, ELISA

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 724(3):376-389.e8., URL: https://www.ncbi.nlm.nih.gov/pubmed/30661959,

Store at 2-8°C. Do not freeze.