Anti-Hu Granzyme A Purified

Granzyme A is a serine protease expressed in the cytoplasmic granules of T cells and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Similarly to granzyme B, granzyme A acts to destroy the target cells by proteolysis of their particular components. In case of granzyme A the targets are e.g. APEX1 (it destroys its oxidative repair activity), and nucleosome assembly protein SET (it disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA).

The mouse monoclonal CB9 recognizes granzyme A, a 28 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

RUO

Mouse

Human

Full length human granzyme A

Granzyme A

Igg1 κ

CB9

Flow cytometry: Recommended dilution: 5-10 μg/mL, intracellular staining.

ICC, FC, IP

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Choi BD, Gedeon PC, Herndon JE, Archer GE, Reap EA, Sanchez-Perez L, Mitchell DA, Bigner DD, Sampson JH: Human regulatory T cells kill tumor cells through granzyme-dependent cytotoxicity upon retargeting with a bispecific antibody. Cancer Immunol Res. 2013 Sep1(3):163. , URL: https://pubmed.ncbi.nlm.nih.gov/24570975/,*Beresford PJ, Kam CM, Powers JC, Lieberman J: Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them. Proc Natl Acad Sci U S A 1997 Aug 1994(17):9285-90 , URL: https://pubmed.ncbi.nlm.nih.gov/9256474/,*Mahrus S, Craik CS: Selective chemical functional probes of granzymes A and B reveal granzyme B is a major effector of natural killer cell-mediated lysis of target cells. Chem Biol. 2005 May12(5):567-77. , URL: https://pubmed.ncbi.nlm.nih.gov/15911377/,*Bratke K, Kuepper M, Bade B, Virchow JC, Luttmann W: Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005 Sep35(9):2608-16, URL: https://pubmed.ncbi.nlm.nih.gov/16106370/,

Store at 2-8°C. Do not freeze.