Why is FITC used for Excitation and Emission in Flow Cytometry?

In flow cytometry, laser light is usually used to excite the fluorochromes. These lasers produce light in the UV and/or visible range. Fluorochromes are selected based on their abilities to fluoresce with the wavelengths of light produced by the lasers. The electrons of a fluorochrome can be excited by a range of wavelengths of light. This optimal wavelength is called the excitation peak. Similarly, the light produced by fluorochromes has a range of wavelengths and this optimal wavelength is called Emission Peak.

What is Fluorescence-activated cell sorting (FACS)?

Fluorescence-activated cell sorting (FACS) is a specialized type of lymphocyte flow cytometry. It provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. It is a useful scientific instrument, as it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest. BD FACSCalibur or FACSCanto are the most use sorters.

Kronos HT WSL-1565 Installed By Gentaur Team

Gentaur experts installed the Kronos HT WSL-1565 Luminometer in cooperation with technical staff of the Vlaams Instituut voor Biotechnologie Laboratory.Kronos HT dispositif is a bioluminescence measurement system for living cell and tissues
Kronos Dio is performing to detect high sensitivity for dim and weak luminescence consecutively in culture samples such as cells and tissues in 35mm culture dishes for a long time.