Quantitative sandwich ELISA for measuring Bovine DNA-directed RNA polymerase II subunit RPB3 (POLR2C) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:POLR2C encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21. The deduced 275-amino acid human POLR2C protein, termed RPB33 by the authors, shares 45% sequence identity with the yeast homolog RPB3. POLR2C contains a single cysteine-rich region. Northern blot analysis of HeLa cell mRNA detected an approximately 1.8-kb POLR2C doublet.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.