ELISA kit for Human CTGF (Connective Tissue Growth Factor)
Seller Gentaur · Catalog number: ELK1246
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Availability:
Made to order
Price
0.01 USD*
Size
1 plate of 96 wells
* Net price, excluding transport costs. Contact us to receive the current offer within 24 hours.
A sandwich ELISA kit for detection of Connective Tissue Growth Factor from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
- Specifications:
- Species reactivity: Human
- Sensitivity: 3.5pg/mL
- Detection range: 7.8-500pg/mL
- Standard solution concentration: 500pg/mL
- ELISA type: Sandwich
- Assay length: 3h
- Storage and shipping:The Human CTGF ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Notes:This kit is intended for research use only. Do not use for diagnostics purposes.
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Additional informations:
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connective Tissue Growth Factor (CTGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connective Tissue Growth Factor (CTGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connective Tissue Growth Factor (CTGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of Connective Tissue Growth Factor (CTGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.