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ELISA kit for Human HPRT1 (Hypoxanthine Phosphoribosyltransferase 1)

CAT:ELK3546Size:1 plate of 96 wellsPrice:Ask for quotation
Availability:
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Shipping:
This product ships without dry ice.



ELISA kit for Human HPRT1 (Hypoxanthine Phosphoribosyltransferase 1)

Product Description:

A sandwich ELISA kit for detection of Hypoxanthine Phosphoribosyltransferase 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Specifications:

Species reactivity: Human

Sensitivity: 0.059ng/mL

Detection range: 0.156-10ng/mL

Standard solution concentration: 10ng/mL

ELISA type: Sandwich

Assay length: 3h

Storage and shipping:

The Human HPRT1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

Additional informations:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &plusmn

10nm. The concentration of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Notes:

This kit is intended for research use only. Do not use for diagnostics purposes.