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ELISA kit for Human HPRT1 (Hypoxanthine Phosphoribosyltransferase 1)
ELISA kit for Human HPRT1 (Hypoxanthine Phosphoribosyltransferase 1)
Product Description:A sandwich ELISA kit for detection of Hypoxanthine Phosphoribosyltransferase 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Species reactivity: Human
Sensitivity: 0.059ng/mL
Detection range: 0.156-10ng/mL
Standard solution concentration: 10ng/mL
ELISA type: Sandwich
Assay length: 3h
The Human HPRT1 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
10nm. The concentration of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This kit is intended for research use only. Do not use for diagnostics purposes.