Quantitative sandwich ELISA for measuring Human L-Glucosamine--fructose-6-phosphate aminotransferase (GFAT) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:Glutamine:fructose-6-phosphate amidotransferase (GFAT) catalyzes the formation of glucosamine 6-phosphate and is the first and rate-limiting enzyme of the hexosamine biosynthetic pathway. GFAT controls the flux of glucose into the hexosamine pathway, and thus formation of hexosamine products, and is most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Expression of these cDNA in E. coli produced a protein of approximately 77 kD and increased GFAT activity 4.5-fold over endogenous bacterial levels. Recombinant.GFAT1L protein possessed functional GFAT activities and biochemical characteristics similar to those of GFAT1. Previously, GFAT1 was considered a simplex enzyme.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.