Quantitative sandwich ELISA for measuring Human Left-right determination factor 1 (LEFTY1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:Vertebrates exhibit numerous left-right (L-R) asymmetries, such as the position of the heart and spleen on the left side. L-R polarity is determined early during development, around the presomite stage in mammals. Several genes have been shown to be expressed asymmetrically relative to the L-R axis. Meno et al. (1996, 1997) identified 2 transforming growth factor-beta-related genes, Lefty1 and Lefty2, that are expressed on the left side of developing mouse embryos.Kosaki et al. (1999) characterized 2 homologs of the murine Lefty1 and Lefty2 genes, which they designated LEFTY B and LEFTY A, respectively. Both genes encode proteins with 366 amino acids. The deduced amino acid sequences of LEFTY B and LEFTY A are more similar to each other than to mouse Lefty1 or Lefty2.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.