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ELISA kit for Rat PDCN (Podocin)
ELISA kit for Rat PDCN (Podocin)
Product Description:A sandwich ELISA kit for detection of Podocin from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Species reactivity: Rat
Sensitivity: 0.056ng/mL
Detection range: 0.156-10ng/mL
Standard solution concentration: 10ng/mL
ELISA type: Sandwich
Assay length: 3h
The Rat PDCN ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocin (PDCN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Podocin (PDCN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Podocin (PDCN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
10nm. The concentration of Podocin (PDCN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This kit is intended for research use only. Do not use for diagnostics purposes.