Anti-Galectin-3 Purified

Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.

The rat monoclonal antibody M3/38 recognizes an epitope within amino acids 40-100 in N-terminal domain of murine galectin 3, a 35 kDa lectin expressed both intracellularly, and after its secretion also on the cell surface.

RUO

Rat

Human, Mouse

murine galectin-3

Galectin-3

IgG2a κ

M3/38

Flow cytometry: Recommended dilution: 2-6 μg/mL. Extracellular and intracellular staining.

FC, WB, IHC-P, ICC, IP, FC-IC

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Fogel S, Guittaut M, Legrand A, Monsigny M, Hébert E: The tat protein of HIV-1 induces galectin-3 expression. Glycobiology. 1999 Apr9(4):383-7., URL: https://pubmed.ncbi.nlm.nih.gov/10089212/,*Sundqvist M, Welin A, Elmwall J, Osla V, Nilsson UJ, Leffler H, Bylund J, Karlsson A: Galectin-3 type-C self-association on neutrophil surfaces The carbohydrate recognition domain regulates cell function. J Leukoc Biol. 2018 Feb103(2):341-353., URL: https://www.ncbi.nlm.nih.gov/pubmed/29345346,*Nachtigal M, Al-Assaad Z, Mayer EP, Kim K, Monsigny M: Galectin-3 expression in human atherosclerotic lesions. Am J Pathol. 1998 May152(5):1199-208., URL: https://www.ncbi.nlm.nih.gov/pubmed/9588889,

Store at 2-8°C. Do not freeze.