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ELISA kit for Human IL9 (Interleukin 9)
ELISA kit for Human IL9 (Interleukin 9)
Product Description:A sandwich ELISA kit for detection of Interleukin 9 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Species reactivity: Human
Sensitivity: 6.1pg/mL
Detection range: 15.6-1000pg/mL
Standard solution concentration: 1000pg/mL
ELISA type: Sandwich
Assay length: 3h
The Human IL9 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 9 (IL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 9 (IL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 9 (IL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
10nm. The concentration of Interleukin 9 (IL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
This kit is intended for research use only. Do not use for diagnostics purposes.