Mouse Anti-Bovine Rhodopsin Monoclonal IgG1

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).
Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.

OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody

Rhodopsin Antibody, Clone 4D2: ATTO 488

12352203

509933

P02699

NP_001014890.1

Membrane

Bovine Rhodopsin

Rhodopsin

4D2

ATTO 488

Protein G Purified

1 µg/ml of SMC-176 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

1 mg/ml

WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.

0.1

640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61.
2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867.
3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875.
4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742.
5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

https://www.stressmarq.com/wp-content/uploads/SMC-176_Rhodopsin_Antibody_4D2_IHC_Mouse_retina_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-176_Rhodopsin_Antibody_4D2_WB_Bovine_photoreceptor-membranes_1.png | https://www.stressmarq.com/wp-content/uploads/SMC-176_Rhodopsin_Antibody_4D2_WB_Human_A549-cells_1.png

https://www.stressmarq.com/wp-content/uploads/2015/05/ATTO-488-Fluorescent-conjugate.pdf

https://www.stressmarq.com/wp-content/uploads/Antibody-MSDS.pdf