Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

ARVD11; Cadherin family member 2; CDHF2; Desmocollin-2; Desmocollin-3; Desmosomal glycoprotein II;III; Desmosomal glycoprotein II; Desmosomal glycoprotein III; DG2; DGII/III; DSC2; DSC3

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

DSC2

1824

95612

Q02487

Plasma membrane

Human desmocollin-2 extracellular domain (exact sequence is proprietary)

Desmocollin-2/3

7G6

CF740

Animal

IHC, FFPE (verified)

Cell adhesion

HeLa cells. Skin

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

110 kDa (DSC2); 100 kDa (DSC3)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

https://cdn.gentaur.com/products/extension/v_datasheet/PI-Protocols-for-Antibody-Based-Detection.pdf

https://cdn.gentaur.com/products/extension/v_msds/SDS-primary-antibodies.pdf