Recognizes a homodimeric protein comprised of 50kDa subunits, identified as Semaphorin-4D, or CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Q92854

PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

Functional studies, FACS, IF

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Store the Semaphorin-4D antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This Semaphorin-4D antibody is available for research use only.