IgA Technique

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Application For in vitro use. Gentaur Products IgA reagent is used for the quantitative determination of immunoglobulin A (IgA) in human serum and plasma in clinical chemistry system.

Summary and principle of the assay

The IgA assay represents 10 to 15% of serum immunoglobulin. Although it is not certain of its exact role in the serum, the one it plays in the resistance to infections by preventing the adhesion of bacteria, or by preventing the attachment and penetration of viruses.

Importance in recurrent infections and anaphylactic reactions

IgA may be important in recurrent infections and anaphylactic reactions to transfusions. Its increase is also associated with chronic liver disorders, chronic infections, neoplasias of the lower gastrointestinal tract and inflammatory bowel disease.

IgA facilitates the diagnosis

The facility in the diagnosis of ataxia telangiectasia, in the differentiation of M components of multiple myelomas, and in the evaluation of myeloma progression. The decrease in levels is found in isolated genetic deficiencies, combined immunodeficiency disorders, in non-IgA multiple myeloma or in macroglobulinemia

Principle of the Method Quantitative immunoglobulin A (IgA) assay

Principle of the Method Quantitative immunoglobulin A assay is performed with Gentaur Chemistry Products reagent in combination with Calibrator Kit Calibration Sample Kit and Diluent. Pack 2 BSA / Saline Solution

Gentaur Chemistry Products & reagents

Gentaur Chemistry Products IgA reagent is a dual compartment cartridge containing ready-to-use liquid reagents. Clinical specimens, calibration and control samples are automatically diluted with saline solution and mixed with Reagent 1 which contains a polymer. The addition of specific antisera to human (Reagent 2) produces an immunochemical reaction that results in the formation of antigen / antibody complexes.

Antigen / Antibody proportion

The light scattering properties of the antigen / antibody complexes increase the turbidity of the solution, in proportion to the concentration in the sample.

Turbidity wavelength

Turbidity is measured spectrophotometrically at 340 nm. Once calibration has been performed on each batch of reagents, the IgA concentration of each sample to be tested can be calculated using the stored calibration curve and the measured absorbance obtained in the assay.

IgA Technique

Summary and principle of the assay

Importance in recurrent infections and anaphylactic reactions

IgA facilitates the diagnosis

Principle of the Method Quantitative immunoglobulin A (IgA) assay

Gentaur Chemistry Products & reagents

Antigen / Antibody proportion

Turbidity wavelength

Tommy Ounas

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