Dengue Virus Detection

Dengue virus is transmitted by diurnal mosquitoes. Therewould have 50 million cases of dengue in the world every year. For several decades, the dengue is constantly increasing in the world. The RT-PCR test is one of the tests that would allow direct diagnosis from the early phase of the disease.

Adenovirus Antibody

The dengue virus belongs to the genus Flavivirus of the family Flaviviridae. There are four
dengue virus serotypes: DEN-1, DEN-2, DEN-3 and DEN-4.
Immunity against a given serotype is definitive with a neutralizing antibody response.
Infection with a serotype only provides transient immune protection against
other serotypes.

The prevention of dengue fever

The prevention of dengue fever is difficult and is based on several axes:
• constant monitoring of endemic foci and monitoring of epidemics;
• vector control with control of the population of vector vectors responsible fortransmission to humans;
• securing health products of human origin.

western-blot
cell-culture

Viral isolation by cell culture is made from sera obtained between the 1st and the 5th day
of the disease, detection of the virus can be carried out by isolation from continuous lines of
mosquito cells.
The tests used for the detection of NS1 antigen, fast and achievable in most
laboratories, allow the detection of NS1 antigen in the serum of patients of the 1st
at the 5th day after the appearance of the clinical signs.
The detection of the genome of the virus is done by conventional RT-PCR (Reverse Transcriptase – Polymerase Chain Reaction) and RT-PCR in real time.
The serological diagnosis of dengue is based on the detection of IgM and IgG according to their
kinetics of appearance over time.

Given its performance below the RT-PCR test, the NS1 test should not be
used only in case of epidemic context and if the RT-PCR test can not be performed. In these
If RT-PCR is not available, NS1 antigen detection tests can then be performed.
useful for routine diagnosis at the point of care (given their positive predictive value
high). On the other hand,NS1 ELISA tests, and a fortiori NS1 ICT tests, have a predictive value
limited negative.

pcr
Anti-Human Antibody

Clinical cituations

Suspicion of dengue usually occurs in two clinical situations:
• suggestive symptomatology in a patient returning from an area affected by dengue fever;
• suggestive symptomatology in a patient in one of the zones of activity of the vector during the period of activity of the vector as defined each year in the national anti-dissemination plan.

Results

A negative result of the search for NS1 antigen excluding
the diagnosis of dengue, the diagnostic strategy should be continued by:
• RT-PCR until the 7th day after the appearance of clinical signs;
serology (IgM / IgG) from the 5th day after the appearance of clinical signs.

IgM Antibody
green-fluorescent-protein-microscopy

Early diagnosis of dengue allows

• stopping the diagnosis of dengue fever (with caution in endemic areas where several infections occur at the same time);
• better treatment management: treatment and monitoring of patients;
• the introduction of adapted sanitary measures

Tommy Ounas

Tommy Ounas

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