Viral isolation by cell culture is made from sera obtained between the 1st and the 5th day
of the disease, detection of the virus can be carried out by isolation from continuous lines of
The tests used for the detection of NS1 antigen, fast and achievable in most
laboratories, allow the detection of NS1 antigen in the serum of patients of the 1st
at the 5th day after the appearance of the clinical signs.
The detection of the genome of the virus is done by conventional RT-PCR (Reverse Transcriptase – Polymerase Chain Reaction) and RT-PCR in real time.
The serological diagnosis of dengue is based on the detection of IgM and IgG according to their
kinetics of appearance over time.