Measles IgM antibodies Detection

The diagnosis is based on the measurement of IgM anti-measles antibodies in the blood and cerebrospinal fluid. The solid phase enzyme immunoassay (ELISA) is based on the μ-capture technique.

Measle Symtoms

Measles Symptoms

The disease is usually seen in preschool age. The incubation is about 10 days after the contagion. The invasion lasts 4 days and the signs of the disease are as follows:

High fever;
Edema of the eyelids;
Puffy eyes;
Runny nose ;
Abdominal pain ;
Vomiting, etc.

Enzyme immunoassay for determination
qualitative and quantitative IgM antibodies against measles
in human serum and plasma.

Elisa Principle

Principle of Elisa

The solid phase enzyme immunoassay (ELISA) is based on the μ-capture technique.

Elisa Precautions for employment

1. Only intended for in-vitro diagnosis and professional use.
2. Read the instructions completely and carefully before starting the test.
3. In the event of significant damage to the kit packaging, please contact your supplier
4. Follow the lot number and the expiry date.
7. Chemical reagents must be treated as material in agreement with national safety guidelines
8. Cleaning staff must be trained by professionals with regard to risks.
9. Avoid contact with the stop solution.
10. All reagents in this kit containing human serum or plasma have been tested

Measles IgM Antibodies

Elisa Storage and statability

The kit is sent at room temperature and should be stored between 2 ° C and 8 ° C. To be kept safe from
heat or direct light. The storage and stability of the samples and reagents prepared are
indicated in the corresponding chapters.
The microplate arrays are stable until the expiry date of the kit being stored between
2 ° C and 8 ° C in the bag already opened, but hermetically closed.

Materials required for Elisa

Pipettes (Multipette Eppendorf or similar material, CV

Elisa required material
Zika Virus Detection Methodology

Notes for procedure

1. Any improper handling of samples or modification of the test procedure may influence the results.
2. Once the test has started, all steps must be followed without interruption.
3. Avoid contamination of reagents, pipettes and wells / tubes. Always
close the unused vials.

Tommy Ounas

Tommy Ounas

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