Anti-Rt CD8b FITC

The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.

RUO

Mouse

Rat

CD8 positive Wistar rat splenic T cell hybridomas

CD8b

Igg1 κ

341

Flow cytometry: Recommended dilution: 1-4 μg/mL.

FC

0.5 mg/mL

FITC

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

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Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.