CH (Cholesterol) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CH in the samples is then determined by comparing the OD of the samples to the standard curve.

CH

Metabolic pathway; Hepatology; Gastroenterology; Nutrition metabolism; Hormone metabolism.

Competitive Inhibition ELISA

2.5h

500 µg/mL

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

7.82-500 µg/mL

2.41 µg/mL

https://www.elkbiotech.com/pro/ELK8282