Quantitative sandwich ELISA for measuring Mouse Lymphocyte antigen 75 (LY75) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

• Additional information:The LY75 protein has 77% overall amino acid identity with mouse Dec205. LY75 is a member of the MMR family of multidomain molecules. It contains a signal peptide, a cysteine-rich domain, a fibronectin type II domain, 10 carbohydrate recognition-like domains, a transmembrane domain, and a cytoplasmic tail. Northern blot analysis of hematopoietic cell lines revealed the presence of a predominant 7.8-kb and a secondary 9.5-kb transcript in myeloid, B-cell, and Hodgkin cell lines but not in the Jurkat T-cell line. In peripheral blood, immature DC contained barely detectable transcripts, which increased 50-fold upon differentiation and activation.LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.
• Storage and shipping:All of the components are shipped on ice packs/blue ice. Upon receiving the kit keep it at +4 degrees Celsius for up to 1 year (refer to the label on the box for the exact expiration date).
• Notes:This kit is intended for research use only. It should not be used for human or animal clinical diagnostics or treatment.