This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3GlcNAcbeta). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Lewis Y antigen

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

Not Applicable

Not Applicable

Cytoplasmic|Plasma membrane|Secreted (extracellular)

Live Ls174T cells (human colon carcinoma cell line)

Lewis-Y

A70-A/A9

CF740

Animal

IHC, FFPE (verified)

Cancer

Lung, Colon, Bladder or Hepatocellular Carcinoma

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

Multiple

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

https://cdn.gentaur.com/products/extension/v_datasheet/PI-Protocols-for-Antibody-Based-Detection.pdf

https://cdn.gentaur.com/products/extension/v_msds/SDS-primary-antibodies.pdf