EXIPROGEN

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ExiProgen is a breakthrough in synthetic biology allowing for the synthesis and purification of one to 16 proteins per run. 
DNA, in the form of plasmid or linear PCR product, is added to the system and Gentaur’s coupled transcription/translation E. coli extract then synthesizes the protein. 
The crude protein extract is further purified, using His-Tag engineered into the proteins. 
The net result is up to 100 ug of >90% pure Protein in about 6 hours. 
ExiProgen can also purify Nucleic Acids from several sources, making it one of the most versatile systems available.

 

Features and Benefits

Built in protocols optimized for protein synthesis/purification and the extraction of a wide variety of nucleic acid samples
ExiProgen has more contains over 900 protocols, each optimized for protein synthesis/purification and target nucleic acid type and source sample. This optimization enables the user to obtain reproducible results for every run, every day. The instrument software can also be upgraded through the network connection port so you can stay up-to-date with the best performing protocols

 

figure2Cooling Block 
ExiProgen has a built-in cooling block where the elution tube rack sits. 
Sample integrity is ensured by keeping the samples below 10°C.
This allows for overnight runs and provides you with confidence in your results.






figure3Magnetic block & Heating block
To increase extraction efficiency, the ExiProgen has an integrated combined magnetic/heating block. The combination of bead magnetization and sample heating reduces experiment time and increases elution efficiency, resulting in increased sample yield. The heating block’s precision temperature control also ensures reproducible results for protein synthesis reactions.







figure4Contamination Shield
ExiProgen comes with a contamination shield designed to protect the assay from cross-contamination during instrument operation. Any time the pipette tips are moving, the contamination shield will slide under the tips, therefore eliminating the possibility of intra-assay cross-contamination which is a must when working with multiple samples.







figure4Easy to use LCD Touch screen 
The 3.5" touchscreen maximizes efficiency by offering an intuitive interface with simple push-button operation for processes such as selecting protocols and controlling the UV sterilization lamp.








figure3UV lamp
ExiProgen has a powerful UV sterilization lamp that enables the user to sterilize the instrument chamber before and/or after every nucleic acid extraction or protein purification run. This prevents possible inter-assay cross-contamination that may occur on a busy work day.







Experimental Procedure

figure6

Principle of protein synthesis and purification

ExiProgen Protein Synthesis Kit useds a E. coli extract to effect coupled transcription/translation of input DNA, which can be plasmid, or PCR generated DNA. The protein itself is generated with a His-Tag, which is then purified using the Ni-NTA magnetic bead provided. The result is high yields of protein that is >90% pure. 

figure7

 

Nucleic acid extraction principle

ExiProgen DNA/ RNA Kits work on the principle of cell lysis, followed by bind, wash elute from silica magnetic beads. High yields of ultrapure DNA or RNA are obtained with OD260 readings of > 1.8 for DNA and 2.0 for RNA. 

figure8

 

 

Price: 20 250 USD

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