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Benchkote

Benchkote® and Benchkote Plus™
Benchkote 
Benchkote is an absorbent, impermeable material designed to protect laboratory surfaces against hazardous spills. The material features a high quality, smooth, absorbent Whatman paper which quickly absorbs liquid spills and a laminated polyethylene layer that prevents flow through to the working surface. After use the sheet is incinerated or disposed of according to local regulations. This makes both Benchkote and Benchkote Plus excellent products that comply with OSHA Regulation 29CFR 1910.1030 for Occupational Exposure to Bloodborne Pathogens. 

Benchkote Plus 
Benchkote Plus is a thicker, more absorbent material for more demanding applications and can absorb in excess of 0.75 litres of water per square meter.

Features and Benefits

  • Material is very strong, making it tear resistant, wet or dry
  • Smooth white surface can be written on with ink or pencil and lies flat
  • Suitable for saturation with disinfectant to protect benches where pathogens and other bacteria are present
  • Use polyethylene side up to collect deposits without absorption
  • Paper side quickly absorbs liquids preventing liquids from going through to the working surface
  • Spillages are trapped in the absorbent paper
  • Benchkote can be picked up and burnt very easily after use; the polyethylene layer does not melt or drip but is rapidly consumed in the flames

Applications

  • Containing radiochemical spillage and avoiding contamination
  • Recovering spillage of expensive materials
  • Protecting hard surfaces to lessen impact
  • Water or solvent wick for humidity chambers
  • Lining of chemical cabinets, laboratory bench drawers and laboratory hoods
Ordering Information
Catalogue Number Description Dimensions Quantity/Pack
Benchkote
2300-916 Sheets 46 cm x 57 cm 50
2300-594 Pads 46 cm x 57 cm 50
2300-731 Reel 46 cm x 50 m 1
2300-772 Reel 92 cm x 50 m 1
Benchkote Plus
2301-6150 Sheets 50 cm x 60 cm 50
2301-6160 Reel 60 cm x 50 m 1
 
 
 

CryptTest™ membrane filter cartridge

CryptTest™ membrane filter cartridge
> CrypTest membrane filter cartridge
Whatman introduces a new, convenient membrane filter cartridge for the concentration and recovery of water-borne protozoan cysts and oocysts which has been approved by the EPA for Method 1623, Cryptosporidium and Giardia in Water by Filtration. 

The CrypTest Cartridge captures protozoan cysts and oocysts from sample water pumped through the disposable cartridge contained in a re-usable housing. The CrypTest contains a 1.0µm pore size Nuclepore® polycarbonate track etch membrane.

Protozoan cysts and oocysts are recovered from the membrane cartridge by using a simple backwashing procedure. The eluant, containing cysts and oocysts, is decanted from the housing into a collection vessel.

Further concentration, purification and separation by centrifugation, immunomagnetic separation or flow cytometry/cell sorting is then performed. Enumeration and confirmation of the cysts and oocysts is done using epifluorescence and DIC procedures.

Features and Benefits

  • Nuclepore polycarbonate track etch membrane provides total surface capture
  • Simple backwashing procedure yields consistently high recovery
  • Convenient for field sampling-sample water pumped through disposable cartridge contained in durable, reusable housing
  • Simple to use
Method
The CrypTest Cartridge was developed for efficient concentration and recovery of protozoan cysts and oocysts in water. The following method is recommended for processing water samples.

Materials and Equipment

  • CrypTest Cartridge - Catalogue Number 610064
  • Cartridge housing, AMETEK 5" clear polycarbonate - Catalogue Number 71503
  • 1/2" male pipe threads with male hose connectors (2)
  • Laboratory tubing, Tygon® formula R-3603, or equivalent, to which cysts/oocysts will not adhere
  • Flow control valve - 2 litres/minute (0.5 gallons/minute)
  • Water flow meter
  • Peristaltic pump
  • Ultrasonic bath

Elution Buffer

  • NaCl - 8.0g
  • KH2PO4 - 0.2g
  • Na2HPO4 (12H2O) - 2.9g
  • KCl - 0.2g
  • Sodium lauryl sulphate (SDS) - 0.2g
  • Tween 80 - 0.2ml
  • Antifoam A* - 0.02ml
  • Adjust volume to 1 litre with reagent water.
  • Adjust pH to 7.4 with 1 N NaOH or HCl.

*Sigma Chemical Co - Catalogue Number A5758.

CryptTest™ membrane filter cartridge
> Sampling from Carboy

Sample Filtration

Key
A - Sample
B - Filter Inlet
C - Filter Outlet
D - Peristaltic Pump
E - Flowmeter
F - To Waste

1. Clean and rinse cartridge housing 
2. Attach male pipe threads to inlet and outlet of cartridge housing cap. 
3. Attach new or well-cleaned tubing to male hose connectors on inlet and outlet of cartridge housing. Secure both with band clamps. 
4. Install the cartridge filter in the housing. 
5. When filtering sample water from a carboy, thread the outlet tubing through the peristaltic pump and immerse the open end of the inlet tubing in the carboy. Alternatively attach open end of inlet tubing directly to feed line. 
6 Turn on pump and adjust the flow rate to 2 litres/min. 
7 Turn off the pump when the desired sample volume has been filtered.

CryptTest™ membrane filter cartridge
> Sampling from Feed Line

Key
A - Sample Tap
B - Pressure Regulator & Gauge
C - Filter in Housing
D - Flow Totalizer
E - Flow Control Valve
F - Drain Hose

Backwashing the Cartridge to Elute Cysts/Oocysts

1 Detach tubing from housing inlet and decant any water remaining in the inlet side of the housing into a 1 litre sample bottle. 
2 Attach the housing outlet to the discharge tubing of the peristaltic pump. Place the free end of this tubing into a beaker of elution buffer. 
3 Start the pump and run at approximately 400ml/min until the filter outlet core and the housing inlet cavity are filled (approximately 300ml). 
4 Cap the housing inlet and outlet and place the housing in the ultrasonic bath for 2 minutes. 
5 Remove the inlet cap and decant the liquid from the inlet side into the collection bottle. 
6 Repeat steps 3, 4 and 5 and then proceed to step 7. 
7 Connect housing outlet to regulated compressed air source and gradually increase pressure to 5 to 10psi as necessary to drive remaining elution buffer from the outlet side to the inlet side and into the collection bottle. 
After backwashing the cartridge and collecting the eluate, further purification, detection and enumeration of cysts/oocysts may be performed using EPA recommended methods.<

CryptTest™ membrane filter cartridge
> Backwash

Key
A - Eluting Solution
B - Peristaltic Pump
C - Filter Outlet
D - Filter Inlet

Cleaning the Cartridge Housing

The following procedure is recommended for cleaning the cartridge housing between samples.

1. Wash thoroughly with water and laboratory detergent, using a brush to clean all crevices. 
2. Soak in 6% sodium hypochlorite solution for 30 minutes. 
3. Rinse thoroughly with tap water. 
4. Rinse with laboratory water. 
 

 
 

pH Indicators and test papers

pH Indicators and test papers
Whatman offers a range of pH indicator and test papers to meet your specific needs. Made with traditional Whatman quality, these products combine ease-of-use with unsurpassed accuracy and consistency. 

The convenience of using indicator papers for the rapid determination of pH values has led to many applications in laboratories and industry.

Features and Benefits

  • Instant pH readings
  • Accurate for a wide range of routine pH testing
  • Inexpensive
  • Convenient and portable for field use

pH Indicators

Strips - Type CF 
Individual plastic support strips carry four different segments of dye-impregnated indicator papers. The resulting combination of colour differences gives an extremely clear and accurate visual pH value. All the dyes are chemically bonded to the paper and cannot be leached into solution; problems associated with contamination of the sample and resultant anomalous readings are avoided.

Strips Type CS 
Each test strip has a central segment of indicator dye and, printed alongside, 8 or more different colour segments marked with corresponding pH values for matching purposes. The pH test value can be read off by direct comparison of the test strip colour and the colour bars. Ideal for coloured solutions, when any changes in colour of the paper stock are automatically cancelled out.

Dispensers Type TC 
The strip has 3 separate indicator dye colour bands. The unique combination of colour change resulting from each test is compared with the colour-coded comparison chart, printed on the dispenser, giving improved speed and accuracy in reading.

Dispensers Type SR 
A full range and some narrow ranges in this popular pH indicator dispenser.

Indicator Books 
The book format is particularly suitable for educational and industrial use. In schools they are economical because the amount of paper per student can be carefully controlled.

Acid-Alkali Test Papers

Litmus Blue and Litmus Red 
These easy-to-use test papers facilitate a general test for acid or alkaline reaction. The change occurs around pH 5-8. They are particularly recommended for educational use.

Congo Red 
This test paper changes colour from blue to red in the range pH 3-5 for the determination of neutralization point in strong acid/weak alkali reactions.

Phenolphthalein 
This white paper changes to pink at pH 8.3 and becomes red at pH 10. It is useful for the determination of the neutralization point in weak acid/strong alkali reactions.

Specialized Test Papers

Lead Acetate Test Paper 
Used for detecting hydrogen sulphide, this rapid qualitative test paper when wetted with distilled water can detect as little as 5 ppm of H2S in the atmosphere or in a gas stream. Hydrogen peroxide can be detected with this paper by pre-blackening the paper in H2S. Concentrations as low as 4 ppm can be detected.

Potassium Iodide Test Paper 
Used for detecting chlorine and other oxidizing agents. In acid solution, oxidizing agents react with the iodide in the test paper to liberate iodine. The paper will turn blue in the presence of an oxidizing agent (e.g., CI2, Br2, H2O2, HNO2, etc.).

Ordering Information
Catalogue Number Type Description pH Range pH Unit Graduation Dimensions Packaging
pH Indicator Papers
2613-991 CF Color Bonded 0–14 1 6 mm x 80 mm Pack of 100 Strips
2614-991 CF Color Bonded 4.5–10.0 0.5 6 mm x 80 mm Pack of 100 Strips
2612-990 CS Integral Comparison Strip 1.0–12.0 1 11 mm x 100 mm Pack of 200 Strips
2626-990 CS Integral Comparison Strip 1.8–3.8 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2627-990 CS Integral Comparison Strip 3.8–5.5 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2628-990 CS Integral Comparison Strip 5.2–6.8 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2629-990 CS Integral Comparison Strip 6.0–8.1 0.3 11 mm x 100 mm Pack of 200 Strips
2630-990 CS Integral Comparison Strip 8.0–9.7 0.2–0.3 11 mm x 100 mm Pack of 200 Strips
2631-990 CS Integral Comparison Strip 9.5–12.0 0.5 11 mm x 100 mm Pack of 200 Strips
Dispensers
2611-628 TC Three Colors 1.0–11.0 1 10 mm x 5 m Pack of 1 Dispenser
2600-100A SR Standard Full Range 1.0–14.0 1.0–2.0 7 mm x 5 m Pack of 1 Dispenser
2600-101A SR Standard Narrow Range 0.5–5.5 0.5–1.0 7 mm x 5 m Pack of 1 Dispenser
2600-102A SR Standard Narrow Range 4.0–7.0 0.5 7 mm x 5 m Pack of 1 Dispenser
2600-103A SR Standard Narrow Range 6.4–8.0 0.5 7 mm x 5 m Pack of 1 Dispenser
2600-104A SR Standard Narrow Range 8.0–10.0 0.5 7 mm x 5 m Pack of 1 Dispenser

Speciality products
Ordering Information
Catalogue Number Description Packaging
Acid Alkali Test Papers
Dispensers
     
2600-201A Litmus Blue Pack of 1 Dispenser
2600-204A Phenolphthalein  
Books
     
2600-601 Lithmus Blue Carton of 10 packs of 10 Books
2600-602 Litmus Red 20 Leaves per Book
2600-500 pH Indicator Booklet 
pH 1 - 11
10 Strips per Book
10 Books per Pack
10 Packs per Carton
Specialized Test Papers Dispensers
     
2602-500A Potassium Iodide 7 mm x 5 m

Universal Indicator papers

Universal indicator papers have been impregnated with a mixture of several indicators. On contact with the sample solution they assume a particular colour. A check against the colour comparison table supplied allows the pH to be determined.

Ordering Information - PANPEHA – universal indicator papers
Catalogue Number Measuring pH range pH color change Size (mm) Qty/Pkg
PANPEHA
10 362 030 1-11 1 7 mm x 5 m 1
PANPEHA Plus*
10 362 000 0-14 1 6 x 85 100
10 362 010 2-9 0.5 6 x 85 100
PANPEHA Nr. 112
10 360 005 0-14 0.5/1** 10 x 75 200
Litmus blue
10 360 300 8-5 blue-red 10 x 75 100
Litmus red
10 360 400 5-8 red-blue 10 x 75 100
Phenolphthalein
10 360 700 8.3-10 white-red 10 x 75 100

* non-bleeding
** from pH 0-9 in 0.5 pH steps; from pH 9-14 in 1 pH steps

Clinical Papers

Antibiotic assay disks

For determining the type of causal agent of infectious diseases and for checking their sensitivity to antibiotics and chemotherapeutic agents in vitro by means of the inhibition zone determination method. The antibiogram allows rational and selective chemotherapy. The test disks are coated with chemotherapeutic agents, placed on the inoculated nutrient agar and incubated. The size of the inhibition zone is a measure for the effectiveness of the substances.

Papers for clinical applications

  • Grade 557: Tallquist hemoglobin scale (for direct rapid testing of the hemoglobin value in blood).
  • Grade 470: Soft surface. For gelatinous samples. Used for the absorption of culture media, as blotting paper, for electrophoresis and amino acid chromatography.
Ordering Information - Antibiotic assay paper
Catalogue Number Size (mm) Qty / Pkg
Grade 557
10 320 390 95 x 135 1 booklet (250 leaves)
Grade 470
10 318 487 200 x 200 100
10 318 493 460 x 570 100
10 318 489 203 x 305** 25

* corresponds 4 x 51/4 inches
** corresponds 8 x  12 inches

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