Nucleic Acid Sample Preparation

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FTA classic card
 
Nucleic acid sample preparation
> FTA classic card
Whatman has been manufacturing quality paper products since 1740 and is recognised as the world leader in filter separation technologies and products. 

With all this knowledge and remarkable testimonies to quality, Whatman has taken its products to a new level, in the genomics/proteomics industry. Whatman's area of expertise in this evolving industry lies in sample preparation, where our FTA® Cards - a revolutionary patented technology for collecting, transporting, purifying and archiving DNA and RNA all from a single card stored at room temperature - have become market leaders.

The nucleic acid sample preparation products incorporate unique Whatman technologies, which offer several outstanding advantages to molecular biologists. These include the encapsulation of solid media into devices, DNA separation products, services and products designed for the collection, transportation, purification and analysis of nucleic acids. All of these new Whatman products create breakthrough applications that yield accurate results much faster than previously possible. Offering an extensive, leading-edge product range and an efficient contract service means that all your DNA processing requirements are met by one established provider.

Whatman offers an extensive range of products to facilitate genomic studies of humans, animals, plants and microorganisms. Collection, storage and analysis of DNA and RNA all benefit from the use of FTA and other Whatman tools.

 

3MM Chr and 17 Chr Blotting Papers

Blotting papers
3MM Chr and 17 Chr Blotting Papers

3MM Chr Whatman 3MM Chr paper is the world's most widely used blotting paper.

This acceptance and usage reflect the high quality, purity and consistency that are relied upon by researchers doing Southern, Northern and Western transfers. 3MM Chr paper is now available in the most widely used sizes. A medium thickness paper (0.34 mm) used extensively for general chromatography and electrophoresis. Flow rate is 130 mm/ 30 min.

17 Chr 
A thick (0.92 mm) and highly absorbent paper with a very high flow rate of 190 mm/ 30 min. Suitable for the heaviest loadings and ideal for preparative paper chromatography and electrophoresis.

Features and Benefits

  • Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind. Ensures that no contamination will occur during the transfer steps
  • Manufactured and tested specifically for chromatographic techniques. This ensures the wicking capability and uniformity of capillary action that is important in obtaining clean and even transfers during blotting
  • Whatman 3MM Chr is considered the industry standard for blotting procedures
  • Convenient sizes available in sheets precisely cut to the most popular gel and transfer membrane sizes. Allows "out-of-the-box" usage and eliminates sheet-to-sheet variations
Ordering Information - Blotting Paper for Ethanol Cleanup
Catalogue Number Quantity/Case
96166729 25
Ordering Information - 3MM Chr Pure Cellulose Grade Sheets
Catalogue Number Size (cm) Quantity/Packs
3030-6185 11 x 14 100
3030-6132 12 x 14 100
3030-153 15 x 17.5 100
3030-6188 15 x 20 100
3030-221 18 x 34 100
3030-861 20 x 20 100
3030-6461 26 x 41 100
3030-347 35 x 43 100
3030-392 35 x 45 100
3030-335 31.5 x 35.5 100
3030-917 46 x 57 100
3030-931 58 x 68 100
3030-6189 4" x 5.25" 100
3030-6187 6" x 8" 100
3030-6185 11 x 14 100
17 Chr Pure Cellulose Grade Sheets
3017-915 46 x 57 25
 
  • Clone Archiving

CloneSaver™ Card

Clone archiving
> CloneSaver Card
FTA® Technology in 96 Well Format for High Throughput Applications

Designed for the collection, long-term storage and purification of plasmid and BAC DNA from bacterial clones in a 96 well format.

Prepare BAC and Plasmid DNA with Amazing Ease

  • Apply 5 µL bacterial culture, re-suspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing
  • Bacteriophages are inactivated
  • DNA is easily accessible for downstream applications
  • Store up to 96 samples on each card

Store Sample DNA for Years at Room Temperature 
Plasmid DNA stored on CloneSaver Cards is stable at room temperature for at least 4 years...and counting.

DNA is Easily Accessible for Downstream Applications

Transformation 
Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods.

PCR 
Immobilized plasmid DNA on a CloneSaver Card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies.

Sequencing 
Plasmid DNA eluted from a CloneSaver punch can be amplified by rolling circle amplification, such as Amersham Bioscience's TempliPhi™ and then sequenced without the need for culture re-growth and plasmid purification.

Clone archiving
> CloneSaver Card with Multi-Barrier Pouches

CloneSaver™ Resealable Multi-Barrier Pouches

Used for transporting or storing the CloneSaver Card. The pouch is constructed with seven laminated layers that protects the card from exposure to gas or liquid contamination. There is a zip-lock resealable closure for easy access to the CloneSaver Card. The tamper-evident seal maintains sample security and the outer paper surface can be used for labelling or writing.

SPOT CloneSaver/BloodSaver Holder

SPOT is a rigid frame that allows automated spotting to standard CloneSaver and BloodSaver cards. It keeps the card flat for uniform and precise spotting of your biological samples. A 96 well card is easily inserted into the SBS-compatible frame, which can then be placed onto a liquid-handling deck just like a multiwell plate.

Clone archiving
> SPOT Holder
The SPOT holder is compatible with standard liquid handlers manufactured by companies such as Beckman Coulter and Tecan Instruments.

CloneSaver Starter Kit

Includes: 2 CloneSaver Cards, 2 Uni-Core Punches (2 mm) with cutting mat and instructions.

Ordering Information - Clone Archiving
Catalogue Number Description Cards/Pack Sample Areas/Card Maximum Volume/Sample Area (µL) Maximum Total Volume/Card (µL)
WB120052 CloneSaver Starter Kit N/A N/A N/A N/A
WB120028 CloneSaver Card 5 96 well format 5 480
WB100024 CloneSaver Resealable Multi-Barrier Pouch 50 N/A N/A N/A
WB100034 SPOT Holder for Semi-Automated Spotting 1 N/A N/A N/A
 
 

EasyClone 384™ PlateNucleic acid sample preparation

Clone archiving
> EasyClone 384 Plate
EasyClone 384™ Plate
The Whatman EasyClone 384 replaces traditional freezer storage methods and offers first of its kind, single device for entire sample archiving and purification workflow, enabling faster DNA mining and discovery. 

The EasyClone 384 plate can be used by biotechnology, pharmaceutical, government and academic laboratories for the archiving, shipping and purifying of clones - all at room temperature.

Relying on proven Whatman FTA® technology, which allows for the collection, storage and purification of DNA from a variety of biological samples, EasyClone consists of a 384-well storage and extraction plate with a piercable foil bottom and FTA disks pre-cut into each well.  The design and format of the EasyClone 384 plate enables the genomics market to use FTA as a replacement for both ultra-low archiving and purification kits.

Ordering Information - EasyClone 384™ Plate
Catalogue Number Description Quantity/Case
WB120069 EasyClone 384 plates 50
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