Pan Cytokeratin Antibody Cocktail (Acidic + Basic)

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.

Q7Z794; Q01546

Mouse

Human epidermal keratin was used as the immunogen for the Pan Cytokeratin antibody.

Monoclonal

IgG1 κ

FACS, IF, WB, IHC-P

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Aliquot the Pan Cytokeratin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This Pan Cytokeratin antibody is available for research use only.