Phosphorylated-p21 Antibody (pT57)

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

P38936

Rabbit

This phosphorylated-p21 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT57 of human P21CIP1.

Polyclonal

Ig

DB, IHC, ELISA

Antigen affinity purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

Aliquot the phosphorylated-p21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

This phosphorylated-p21 antibody is available for research use only.