Anti-Hu CD41/CD61 (PAC-1 epitope) PE

CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.

The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.

RUO

Mouse

Human

Human platelets

CD41/CD61 (PAC-1 epitope)

IgM κ

PAC-1

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

PE

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

*Shattil SJ, Hoxie JA, Cunningham M, Brass LF: Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. J Biol Chem. 1985 Sep 15260(20):11107-14. , URL: http://www.ncbi.nlm.nih.gov/pubmed/2411729,*Shattil SJ, Cunningham M, Hoxie JA: Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood. 1987 Jul70(1):307-15. , URL: http://www.ncbi.nlm.nih.gov/pubmed/3297204 ,*Trikha M, Timar J, Lundy SK, Szekeres K, Cai Y, Porter AT, Honn KV: The high affinity alphaIIb beta3 integrin is involved in invasion of human melanoma cells. Cancer Res. 1997 Jun 1557(12):2522-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/9192835 ,*Bourne JH, Colicchia M, Di Y, Martin E, Slater A, Roumenina LT, Dimitrov JD, Watson SP, Rayes J: Heme induces human and mouse platelet activation through C-type-lectin-like receptor-2. Haematologica. 2021 Feb 1106(2):626-629., URL: https://pubmed.ncbi.nlm.nih.gov/32354867/,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.