LIG1 Antibody / DNA Ligase 1

DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

P18858

Mouse

Full-length native cow LIG1 protein was used as the immunogen for the DNA Ligase 1 antibody.

Monoclonal

IgG1 κ

WB, ELISA, IP

Purified

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Store the DNA Ligase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This DNA Ligase 1 antibody is available for research use only.