TNF alpha Antibody Cocktail

TNF alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha causes cytolysis of certain transformed cells. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

P01375

Mouse

An N-terminal recombinant protein fragment (TNF706) and amino acids 115-130 (NGVELRDNQLVVPSEG) were used as the immunogen for the TNF-alpha antibody cocktail.

Monoclonal

IgM κ

IHC-P

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the TNF-alpha antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This TNF-alpha antibody cocktail is available for research use only.