Vimentin Antibody

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Mouse

A recombinant human partial protein (amino acids 2-466) was used as the immunogen for this Vimentin antibody.

Monoclonal

IgG2b κ

IHC-P, WB

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This Vimentin antibody is available for research use only.