Anti-Hu CD79a APC

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

The mouse monoclonal antibody ZL7.4 interacts with extracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

RUO

Mouse

Human

IgM complex isolated from Daudi cells

CD79a

Igg1 κ

ZL7.4

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

FC

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Stopforth RJ, Oldham RJ, Tutt AL, Duriez P, Chan HTC, Binkowski BF, Zimprich C, Li D, Hargreaves PG, Cong M, Reddy V, Leandro MJ, Cambridge G, Lux A, Nimmerjahn F, Cragg MS: Detection of experimental and clinical immune complexes by measuring SHIP-1 recruitment to the inhibitory FcγRIIB. J Immunol. 2018 Mar 1200(5):1937-1950., URL: https://pubmed.ncbi.nlm.nih.gov/29351998/,*Bell PB, Rooney N, Bosanquet AG: CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase. Cytometry. 1999 Jun 1538(3):102-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/10397328,*Luger D, Yang YA, Raviv A, Weinberg D, Banerjee S, Lee MJ, Trepel J, Yang L, Wakefield LM: Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects. PLoS One. 2013 Oct 168(10):e76115., URL: https://www.ncbi.nlm.nih.gov/pubmed/24146823,*Cragg MS, Chan HT, Fox MD, Tutt A, Smith A, Oscier DG, Hamblin TJ, Glennie MJ: The alternative transcript of CD79b is overexpressed in B-CLL and inhibits signaling for apoptosis. Blood. 2002 Nov 1100(9):3068-76., URL: https://pubmed.ncbi.nlm.nih.gov/12384401/,

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe JG: Analysis of the individual contributions of Igalpha (CD79a)- and Igbeta (CD79b)-mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1177(11):7913-22., URL: http://www.ncbi.nlm.nih.gov/pubmed/17114463?ordina,*Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1174(3):1245-52., URL: http://www.ncbi.nlm.nih.gov/pubmed/15661879?ordina,*Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15173(2):1000-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15240688?ordina,*Pike KA, Iacampo S, Friedmann JE, Ratcliffe MJ: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15172(4):2210-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/14764688?ordina,*Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe JG: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3194(11):1583-96., URL: http://www.ncbi.nlm.nih.gov/pubmed/11733573?ordina,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.