Pig Estrogen ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.

E ; Estrogen

Reproductive science.

Competitive Inhibition ELISA

2.5h

1000 pg/mL

Serum, plasma, tissue homogenates and other biological fluids

15.625-1000 pg/mL

4.18 pg/mL

https://www.elkbiotech.com/pro/ELK10160