p53 Antibody / TP53

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. Its epitope maps within the N-terminus (aa 20-25) of p53. Monoclonal antibody PAb1801 does not block the binding of DO-7 MAb to p53 in an ELISA test. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.

P04637

Mouse

Recombinant human wild type p53 protein expressed in E. coli was used as the immunogen for the p53 antibody.

Monoclonal

IgG2a κ

FACS, IF, WB, IHC-P

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This p53 antibody is available for research use only.