Anti-Cytokeratin 10(KRT10/844), Biotin conjugate

This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

KRT10

3858

99936

P13645

Cytoskeleton

Mouse

Human,Mouse

Recombinant human KRT10 protein

Cytokeratin 10

Monoclonal

IgG1 κ

KRT10/844

Biotin

Tumor

Animal

IHC, FFPE (verified) | WB (verified)

IHC,FFPE,WB

Cancer, Cytoskeleton

A431, HeLa, MCF7 cells. Esophagus or tonsil.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

56.5 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.1-0.2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Stable at room temperature or 37°C (98°F) for 7 days.

2 years