This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; TIMP2; Tissue Inhibitor of Metalloproteinase 2

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

TIMP2

7077

633514

P16035

Cytoplasmic|Secreted (extracellular)

A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

TIMP2

3A4

CF740

Animal

IHC, FFPE (verified)

Extracellular matrix, Proteases

HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

21 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

https://cdn.gentaur.com/products/extension/v_datasheet/PI-Protocols-for-Antibody-Based-Detection.pdf

https://cdn.gentaur.com/products/extension/v_msds/SDS-primary-antibodies.pdf