Anti-Hu CD9 Alexa Fluor® 488

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

RUO

Mouse

Human

Pre-B cell line NALM-6.

CD9

IgG1

MEM-61

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.

WB,IHC-P,FC

Alexa Fluor® 488

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1167(5):2724-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/11509616?itool=,*Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am J Physiol Cell Physiol. 2007 Nov293(5):C1660-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17855771?itool=,*Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type-1 matrix metalloproteinase-dependent pericellular proteolysis. Mol Biol Cell. 2009 Apr20(7):2030-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/19211836?itool,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,

*Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2104(1):234-9., URL: https://pubmed.ncbi.nlm.nih.gov/17190803/,*Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov455(2):283-96., URL: https://pubmed.ncbi.nlm.nih.gov/17668233/,*Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov98(5):1081-7., URL: https://pubmed.ncbi.nlm.nih.gov/18000614/,*Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 166(19):9557-65., URL: https://pubmed.ncbi.nlm.nih.gov/17018612/,

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.