3-NT (3-Nitrotyrosine) ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.

3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine

Metabolic pathway; Infection immunity; Rheumatology.

Competitive Inhibition ELISA

2.5h

300 ng/mL

Serum, plasma and other biological fluids

4.69-300 ng/mL

1.4 ng/mL