Anti-ALK (Anaplastic Lymphoma Kinase) / CD246 (ALK/1503), CF594 conjugate

The wild-type anaplastic lymphoma kinase (ALK) protein is a 200 kDa transmembrane receptor tyrosine kinase. Its expression is restricted to a few scattered cells in the nervous system (some glial cells and neurons, and a few endothelial cells and pericytes). ALK has been found to be rearranged, mutated, or amplified in a series of tumors. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation, encodes part of the nucleolar phosphoprotein nucleophosmin (NPM), joined to the entire cytoplasmic portion of ALK. As a consequence, the ALK gene comes under the control of the NPM promoter, which induces a permanent and ubiquitous transcription of the NPM-ALK hybrid gene, resulting in the production of a 80 kDa NPM-ALK chimeric protein. This translocation is found in anaplastic large cell lymphomas (ALCL). Reportedly, expression of ALK indicates a better prognosis. Approximately 5%-10% of non-small cell lung carcinomas also express ALK protein, producing a cytoplasmic staining pattern. This MAb also reacts with blood vessels that serves as an internal positive control. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

ALK Tyrosine Kinase Receptor; Anaplastic Lymphoma Kinase; ALK/NPM1 fusion gene

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

ALK

238

654469

Q9UM73

Plasma membrane

Mouse

Human

Recombinant human ALK protein fragment (aa1360-1460) (exact sequence is proprietary)

ALK | CD246

Monoclonal

IgG1 κ

ALK/1503

CF594

Tumor

Animal

WB (verified)

WB

Cancer, Neuroscience, Signal transduction

Anaplastic Large Cell Lymphoma

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

80 kDa (hybrid); 200 kDa (wild type)

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/mouse-monoclonal-anti-alk-cd246-alk1503