Anti-Cytokeratin 8 (KRT8)(KRT8/2115), CF740 conjugate

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ('ring-like, perinuclear') from ductal ('peripheral-predominant') carcinoma of the breast. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

KRT8

3856

533782; 708445

P05787

Cytoskeleton

Mouse

Human

Recombinant full-length human KRT8 protein

Cytokeratin 8

Monoclonal

IgG1 κ

KRT8/2115

CF740

Tumor

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cancer, Cytoskeleton

MCF-7 , HeLa or A431 cells. Skin, colon, lung or breast carcinoma.

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

52.5 kDa

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/cytokeratin-8-monoclonal-mouse-antibody-krt8-2115