Anti-EMI1(EMI1/1176), CF647 conjugate

This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Early Mitotic Inhibitor-1 (EMI1); FBX5; Fbxo31

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

FBXO5

26271

520506

Q9UKT4

Nucleus

Mouse

Human

Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein

EMI1

Monoclonal

IgG2a κ

EMI1/1176

CF647

Animal

IHC, FFPE (verified) | WB (verified)

IHC, FFPE, WB

Cell cycle

HeLa, 293 or HepG2 cells. Ovarian carcinoma.

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

56 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-emi1-emi11176