Anti-CD171 / L1CAM (L1 Cell Adhesion Molecule)(UJ127), CF568 conjugate

Recognizes a cell surface protein of 220-240 kDa, identified as L1 cell adhesion molecule. The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, and adducted thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, and neural migration as well as in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm. This MAb is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin e.g. neuroblastoma and Schwannomas. It does not bind to pediatric or adult brain.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

L1CAM

3897

52818

P32004

Plasma membrane

Mouse

Human

Homogenous suspension of 16 week human fetal brain

CD171 | L1CAM

Monoclonal

IgG1 κ

UJ127

CF568

Tumor

Animal

Flow, surface (published) | IHC, FFPE (published) | IP (published) | WB (published)

FC, IHC, FFPE, IP, WB

Cancer, Cell adhesion, Developmental biology

Neuroblastomas or Schwannomas

0.1 mg/mL

PBS, 0.1% BSA, 0.05% azide

220-240 kDa

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Gynaecol Oncol (2008) 29(1):26-30. (IHC, FFPE; WB) Anticancer Res (2009) 29: 1195-1200. (IHC, FFPE) Mol Biol Cell (2010) 21: 1671-1685. (IHC, FFPE; Flow, IP)

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/monoclonal-anti-cd171-l1cam-l1-cell-adhesion-molecule-uj127-11