Anti-ERCC1 / RAD10 (Tumor Progression Marker)(ERCC1/2683), CF740 conjugate

Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

COFS4; DNA excision repair protein ERCC1; ERCC1; Excision repair cross complementing 1; RAD10; UV20

41116161

Primary and secondary antibodies for multiple methodology
immunostaining detection application

ERCC1

2067

435981

P07992

Nucleus

Mouse

Human

Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

ERCC1 | RAD10

Monoclonal

IgG1 κ

ERCC1/2683

CF740

Animal

HeLa, HepG2 cells. Tonsil.

0.1 mg/mL

PBS, 0.1% rBSA, 0.05% azide

~110 kDa

For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

Room temperature

4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

2 years

Primary

https://biotium.com/product/ercc1-rad10-monoclonal-mouse-antibody-ercc1-2683